5EIX

QUINOLONE-STABILIZED CLEAVAGE COMPLEX OF TOPOISOMERASE IV FROM KLEBSIELLA PNEUMONIAE

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, D, G, J (A, B, G, J)	DNA topoisomerase 4 subunit B,DNA topoisomerase 4 subunit A	polymer	741	83915.1	4	UniProt (R4YHS8) UniProt (R4YE07)	Klebsiella pneumoniae	Topoisomerase IV subunit B,Topoisomerase IV subunit A
2	B, E, H, K (E, C, H, K)	SYMMETRISED E-SITE (PRE-CUT)	polymer	8	2432.6	4		synthetic construct
3	C, F, I, L (F, D, I, L)	SYMMETRISED E-SITE (PRE-CUT)	polymer	12	3655.4	4		synthetic construct
4	M, N, P, R, S... (A, B, G, J, L)	MAGNESIUM ION	non-polymer		24.3	8	Chemie (MG)
5	O, Q, T, X (F, D, I, L)	(3S)-9-fluoro-3-methyl-10-(4-methylpiperazin-1-yl)-7-oxo-2,3-dihydro-7H-[1,4]oxazino[2,3,4-ij]quinoline-6-carboxylic acid	non-polymer		361.4	4	Chemie (LFX)

Sequence modifications

A, B, G, J: 390 - 998 (UniProt: R4YHS8)

PDB	External Database	Details
Met 389	-	initiating methionine
Glu 999	-	linker
Phe 1000	-	linker

A, B, G, J: 1001 - 1490 (UniProt: R4YE07)

PDB	External Database	Details
Gly 1100	Val 100	conflict
Thr 1255	Ser 255	conflict
His 1491	-	expression tag
His 1492	-	expression tag
His 1493	-	expression tag
His 1494	-	expression tag
His 1495	-	expression tag
His 1496	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	12
	Total formula weight	360012.7
Non-Polymers*	Number of molecules	12
	Total formula weight	1639.9
All*	Total formula weight	361652.6

*Water molecules are not included.