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Yorodumi- PDB-1fai: THREE-DIMENSIONAL STRUCTURE OF TWO CRYSTAL FORMS OF FAB R19.9, FR... -
+Open data
-Basic information
Entry | Database: PDB / ID: 1fai | ||||||
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Title | THREE-DIMENSIONAL STRUCTURE OF TWO CRYSTAL FORMS OF FAB R19.9, FROM A MONOCLONAL ANTI-ARSONATE ANTIBODY | ||||||
Components |
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Keywords | IMMUNOGLOBULIN | ||||||
Function / homology | Function and homology information IgG immunoglobulin complex / B cell differentiation / extracellular region / plasma membrane Similarity search - Function | ||||||
Biological species | Mus musculus (house mouse) | ||||||
Method | X-RAY DIFFRACTION / Resolution: 2.7 Å | ||||||
Authors | Lascombe, M.B. / Alzari, P.M. / Poljak, R.J. / Nisonoff, A. | ||||||
Citation | Journal: Proc.Natl.Acad.Sci.USA / Year: 1992 Title: Three-dimensional structure of two crystal forms of FabR19.9 from a monoclonal anti-arsonate antibody. Authors: Lascombe, M.B. / Alzari, P.M. / Poljak, R.J. / Nisonoff, A. #1: Journal: Proc.Natl.Acad.Sci.USA / Year: 1989 Title: Three-Dimensional Structure of Fab R19.9, A Monoclonal Murine Antibody Specific for the P-Azobenzenearsonate Group Authors: Lascombe, M.B. / Alzari, P.M. / Boulot, G. / Saludjian, P. / Tougard, P. / Berek, C. / Haba, S. / Rosen, E.M. / Nisonoff, A. / Poljak, R.J. #2: Journal: J.Biol.Chem. / Year: 1984 Title: Crystallization of the Fab Fragments of Monoclonal Anti-P-Azophenylarsonate Antibodies and Their Complexes with Haptens Authors: Mariuzza, R.A. / Amit, A.G. / Boulot, G. / Saludjian, P. / Saul, F.A. / Tougard, P. / Poljak, R.J. / Conger, J. / Lamoyi, E. / Nisonoff, A. | ||||||
History |
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-Structure visualization
Structure viewer | Molecule: MolmilJmol/JSmol |
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-Downloads & links
-Download
PDBx/mmCIF format | 1fai.cif.gz | 90.6 KB | Display | PDBx/mmCIF format |
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PDB format | pdb1fai.ent.gz | 73.5 KB | Display | PDB format |
PDBx/mmJSON format | 1fai.json.gz | Tree view | PDBx/mmJSON format | |
Others | Other downloads |
-Validation report
Arichive directory | https://data.pdbj.org/pub/pdb/validation_reports/fa/1fai ftp://data.pdbj.org/pub/pdb/validation_reports/fa/1fai | HTTPS FTP |
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-Related structure data
-Links
-Assembly
Deposited unit |
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1 |
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Unit cell |
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Atom site foot note | 1: RESIDUES PRO L 95, PRO L 141, PRO H 158, AND PRO H 200 ARE CIS PROLINES. |
-Components
#1: Antibody | Mass: 23690.041 Da / Num. of mol.: 1 Source method: isolated from a genetically manipulated source Source: (gene. exp.) Mus musculus (house mouse) / References: UniProt: P01837 |
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#2: Antibody | Mass: 23566.316 Da / Num. of mol.: 1 Source method: isolated from a genetically manipulated source Source: (gene. exp.) Mus musculus (house mouse) |
-Experimental details
-Experiment
Experiment | Method: X-RAY DIFFRACTION |
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-Sample preparation
Crystal | Density Matthews: 2.69 Å3/Da / Density % sol: 54.31 % | ||||||||||||||||||||||||||||||||||||||||||
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Crystal grow | *PLUS pH: 7.2 / Method: vapor diffusion / Details: took 9 from original paper | ||||||||||||||||||||||||||||||||||||||||||
Components of the solutions | *PLUS
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-Data collection
Radiation | Scattering type: x-ray |
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Radiation wavelength | Relative weight: 1 |
Reflection | *PLUS Highest resolution: 2.5 Å / Num. obs: 16492 / Observed criterion σ(I): 2 / Num. measured all: 41617 / Rmerge(I) obs: 0.08 |
Reflection shell | *PLUS Highest resolution: 2.5 Å / Lowest resolution: 2.7 Å |
-Processing
Software | Name: X-PLOR / Classification: refinement | ||||||||||||
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Refinement | Rfactor Rwork: 0.189 / Highest resolution: 2.7 Å Details: THE POSITIONS OF MANY LOOPS IN THE CONSTANT REGION AND OF THE THIRD COMPLEMENTARITY-DETERMINING REGION OF THE HEAVY CHAIN (RESIDUES H 101 - H 109) ARE SOMEWHAT ARBITRARY, AS INDICATED BY ...Details: THE POSITIONS OF MANY LOOPS IN THE CONSTANT REGION AND OF THE THIRD COMPLEMENTARITY-DETERMINING REGION OF THE HEAVY CHAIN (RESIDUES H 101 - H 109) ARE SOMEWHAT ARBITRARY, AS INDICATED BY LARGE TEMPERATURE FACTORS. | ||||||||||||
Refinement step | Cycle: LAST / Highest resolution: 2.7 Å
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Refine LS restraints |
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Software | *PLUS Name: X-PLOR / Classification: refinement | ||||||||||||
Refinement | *PLUS Lowest resolution: 6 Å / Num. reflection obs: 9855 / σ(F): 3 / Rfactor Rwork: 0.189 | ||||||||||||
Solvent computation | *PLUS | ||||||||||||
Displacement parameters | *PLUS | ||||||||||||
Refine LS restraints | *PLUS
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