+Open data
-Basic information
Entry | Database: PDB / ID: 6al4 | |||||||||
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Title | CRYSTAL STRUCTURE OF ANTI-CD19 ANTIBODY B43 FAB | |||||||||
Components |
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Keywords | IMMUNE SYSTEM | |||||||||
Function / homology | Function and homology information | |||||||||
Biological species | Homo sapiens (human) | |||||||||
Method | X-RAY DIFFRACTION / SYNCHROTRON / MOLECULAR REPLACEMENT / Resolution: 2.45 Å | |||||||||
Authors | Teplyakov, A. / Obmolova, G. / Gilliland, G.L. | |||||||||
Citation | Journal: Proteins / Year: 2018 Title: Crystal structure of B-cell co-receptor CD19 in complex with antibody B43 reveals an unexpected fold. Authors: Teplyakov, A. / Obmolova, G. / Luo, J. / Gilliland, G.L. | |||||||||
History |
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-Structure visualization
Structure viewer | Molecule: MolmilJmol/JSmol |
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-Downloads & links
-Download
PDBx/mmCIF format | 6al4.cif.gz | 264.4 KB | Display | PDBx/mmCIF format |
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PDB format | pdb6al4.ent.gz | 212.5 KB | Display | PDB format |
PDBx/mmJSON format | 6al4.json.gz | Tree view | PDBx/mmJSON format | |
Others | Other downloads |
-Validation report
Arichive directory | https://data.pdbj.org/pub/pdb/validation_reports/al/6al4 ftp://data.pdbj.org/pub/pdb/validation_reports/al/6al4 | HTTPS FTP |
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-Related structure data
Related structure data | 6al5C 5i15S S: Starting model for refinement C: citing same article (ref.) |
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Similar structure data |
-Links
-Assembly
Deposited unit |
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1 |
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2 |
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3 |
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Unit cell |
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-Components
#1: Antibody | Mass: 23815.260 Da / Num. of mol.: 3 Source method: isolated from a genetically manipulated source Source: (gene. exp.) Homo sapiens (human) / Gene: IGK@ / Production host: Homo sapiens (human) / References: UniProt: Q6P5S8 #2: Antibody | Mass: 25225.055 Da / Num. of mol.: 3 / Fragment: FD,FD,FD,FD Source method: isolated from a genetically manipulated source Source: (gene. exp.) Homo sapiens (human) / Gene: IGH@ / Production host: Homo sapiens (human) / References: UniProt: Q6GMX6 #3: Water | ChemComp-HOH / | |
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-Experimental details
-Experiment
Experiment | Method: X-RAY DIFFRACTION / Number of used crystals: 1 |
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-Sample preparation
Crystal | Density Matthews: 3.47 Å3/Da / Density % sol: 64 % |
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Crystal grow | Temperature: 293 K / Method: vapor diffusion, sitting drop / pH: 9.1 / Details: 20% PEG 3350, 0.2 M NA PHOSPHATE DIBASIC / PH range: 9.1 |
-Data collection
Diffraction | Mean temperature: 100 K |
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Diffraction source | Source: SYNCHROTRON / Site: APS / Beamline: 21-ID-F / Wavelength: 0.9785 Å |
Detector | Type: MARMOSAIC 225 mm CCD / Detector: CCD / Date: Jun 18, 2015 |
Radiation | Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 0.9785 Å / Relative weight: 1 |
Reflection | Resolution: 2.38→50 Å / Num. obs: 80381 / % possible obs: 96.9 % / Observed criterion σ(I): -3 / Redundancy: 5.7 % / Biso Wilson estimate: 52.2 Å2 / Rmerge(I) obs: 0.071 / Net I/σ(I): 22.3 |
Reflection shell | Resolution: 2.38→2.47 Å / Redundancy: 5.5 % / Rmerge(I) obs: 0.563 / Mean I/σ(I) obs: 3.4 / Num. unique obs: 7505 / % possible all: 91.7 |
-Processing
Software |
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Refinement | Method to determine structure: MOLECULAR REPLACEMENT Starting model: 5i15 Resolution: 2.45→20 Å / Cor.coef. Fo:Fc: 0.944 / Cor.coef. Fo:Fc free: 0.93 / SU B: 7.759 / SU ML: 0.173 / Cross valid method: THROUGHOUT / σ(F): 0 / ESU R: 0.297 / ESU R Free: 0.223
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Solvent computation | Ion probe radii: 0.8 Å / Shrinkage radii: 0.8 Å / VDW probe radii: 1.2 Å | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Displacement parameters | Biso mean: 60.3 Å2
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Refinement step | Cycle: LAST / Resolution: 2.45→20 Å
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Refine LS restraints |
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