+データを開く
-基本情報
登録情報 | データベース: EMDB / ID: EMD-6771 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
タイトル | Cryo-EM structure of human respiratory complex I matrix arm | |||||||||
マップデータ | This map was obtained by sub-region refinemet | |||||||||
試料 |
| |||||||||
機能・相同性 | 機能・相同性情報 Complex I biogenesis / protein insertion into mitochondrial inner membrane / blastocyst hatching / protein lipoylation / Mitochondrial Fatty Acid Beta-Oxidation / Protein lipoylation / Respiratory electron transport / respiratory gaseous exchange by respiratory system / cellular response to oxygen levels / iron-sulfur cluster assembly complex ...Complex I biogenesis / protein insertion into mitochondrial inner membrane / blastocyst hatching / protein lipoylation / Mitochondrial Fatty Acid Beta-Oxidation / Protein lipoylation / Respiratory electron transport / respiratory gaseous exchange by respiratory system / cellular response to oxygen levels / iron-sulfur cluster assembly complex / mitochondrial large ribosomal subunit binding / gliogenesis / neural precursor cell proliferation / cardiac muscle tissue development / : / [2Fe-2S] cluster assembly / oxygen sensor activity / oxidoreductase activity, acting on NAD(P)H, quinone or similar compound as acceptor / ubiquinone-6 biosynthetic process / cellular respiration / iron-sulfur cluster assembly / NADH dehydrogenase activity / mitochondrial ribosome / sodium ion transport / mitochondrial translation / NADH:ubiquinone reductase (H+-translocating) / positive regulation of execution phase of apoptosis / apoptotic mitochondrial changes / mitochondrial ATP synthesis coupled electron transport / : / mitochondrial electron transport, NADH to ubiquinone / mitochondrial respiratory chain complex I assembly / proton motive force-driven mitochondrial ATP synthesis / RHOG GTPase cycle / endopeptidase activator activity / acyl binding / NADH dehydrogenase (ubiquinone) activity / acyl carrier activity / cellular response to interferon-beta / quinone binding / electron transport coupled proton transport / negative regulation of intrinsic apoptotic signaling pathway / ATP metabolic process / cellular response to retinoic acid / extrinsic apoptotic signaling pathway / response to cAMP / Mitochondrial protein degradation / substantia nigra development / aerobic respiration / respiratory electron transport chain / reactive oxygen species metabolic process / neurogenesis / regulation of mitochondrial membrane potential / synaptic membrane / fatty acid binding / mitochondrial membrane / apoptotic signaling pathway / regulation of protein phosphorylation / brain development / mitochondrial intermembrane space / negative regulation of cell growth / 2 iron, 2 sulfur cluster binding / fatty acid biosynthetic process / circadian rhythm / positive regulation of protein catabolic process / NAD binding / positive regulation of fibroblast proliferation / FMN binding / nervous system development / 4 iron, 4 sulfur cluster binding / protease binding / response to oxidative stress / mitochondrial inner membrane / electron transfer activity / nuclear body / structural constituent of ribosome / mitochondrial matrix / negative regulation of DNA-templated transcription / neuronal cell body / ubiquitin protein ligase binding / calcium ion binding / protein-containing complex binding / mitochondrion / RNA binding / nucleoplasm / ATP binding / nucleus / metal ion binding / cytosol / cytoplasm 類似検索 - 分子機能 | |||||||||
生物種 | Homo sapiens (ヒト) | |||||||||
手法 | 単粒子再構成法 / クライオ電子顕微鏡法 / 解像度: 3.4 Å | |||||||||
データ登録者 | Gu J / Wu M / Yang M | |||||||||
引用 | ジャーナル: Cell / 年: 2017 タイトル: Architecture of Human Mitochondrial Respiratory Megacomplex IIIIIV. 著者: Runyu Guo / Shuai Zong / Meng Wu / Jinke Gu / Maojun Yang / 要旨: The respiratory megacomplex represents the highest-order assembly of respiratory chain complexes, and it allows mitochondria to respond to energy-requiring conditions. To understand its architecture, ...The respiratory megacomplex represents the highest-order assembly of respiratory chain complexes, and it allows mitochondria to respond to energy-requiring conditions. To understand its architecture, we examined the human respiratory chain megacomplex-IIIIIV (MCIIIIIV) with 140 subunits and a subset of associated cofactors using cryo-electron microscopy. The MCIIIIIV forms a circular structure with the dimeric CIII located in the center, where it is surrounded by two copies each of CI and CIV. Two cytochrome c (Cyt.c) molecules are positioned to accept electrons on the surface of the c state CIII dimer. Analyses indicate that CII could insert into the gaps between CI and CIV to form a closed ring, which we termed the electron transport chain supercomplex. The structure not only reveals the precise assignment of individual subunits of human CI and CIII, but also enables future in-depth analysis of the electron transport chain as a whole. | |||||||||
履歴 |
|
-構造の表示
ムービー |
ムービービューア |
---|---|
構造ビューア | EMマップ: SurfViewMolmilJmol/JSmol |
添付画像 |
-ダウンロードとリンク
-EMDBアーカイブ
マップデータ | emd_6771.map.gz | 21.8 MB | EMDBマップデータ形式 | |
---|---|---|---|---|
ヘッダ (付随情報) | emd-6771-v30.xml emd-6771.xml | 10.9 KB 10.9 KB | 表示 表示 | EMDBヘッダ |
画像 | emd_6771.png | 15.9 KB | ||
アーカイブディレクトリ | http://ftp.pdbj.org/pub/emdb/structures/EMD-6771 ftp://ftp.pdbj.org/pub/emdb/structures/EMD-6771 | HTTPS FTP |
-関連構造データ
関連構造データ | 5xtbMC 6772C 6773C 6774C 6775C 6776C 5xtcC 5xtdC 5xteC 5xthC 5xtiC M: このマップから作成された原子モデル C: 同じ文献を引用 (文献) |
---|---|
類似構造データ |
-リンク
EMDBのページ | EMDB (EBI/PDBe) / EMDataResource |
---|---|
「今月の分子」の関連する項目 |
-マップ
ファイル | ダウンロード / ファイル: emd_6771.map.gz / 形式: CCP4 / 大きさ: 421.9 MB / タイプ: IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES) | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
注釈 | This map was obtained by sub-region refinemet | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
ボクセルのサイズ | X=Y=Z: 1.083 Å | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
密度 |
| ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
対称性 | 空間群: 1 | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
詳細 | EMDB XML:
CCP4マップ ヘッダ情報:
|
-添付データ
-試料の構成要素
-全体 : Human respiratory complex I matrix arm
全体 | 名称: Human respiratory complex I matrix arm |
---|---|
要素 |
|
-超分子 #1: Human respiratory complex I matrix arm
超分子 | 名称: Human respiratory complex I matrix arm / タイプ: complex / ID: 1 / 親要素: 0 / 含まれる分子: #1-#18 |
---|---|
由来(天然) | 生物種: Homo sapiens (ヒト) |
-実験情報
-構造解析
手法 | クライオ電子顕微鏡法 |
---|---|
解析 | 単粒子再構成法 |
試料の集合状態 | particle |
-試料調製
濃度 | 0.4 mg/mL |
---|---|
緩衝液 | pH: 7.4 |
凍結 | 凍結剤: ETHANE / チャンバー内湿度: 100 % |
-電子顕微鏡法
顕微鏡 | FEI TITAN KRIOS |
---|---|
撮影 | フィルム・検出器のモデル: FEI FALCON II (4k x 4k) 平均電子線量: 1.25 e/Å2 |
電子線 | 加速電圧: 300 kV / 電子線源: FIELD EMISSION GUN |
電子光学系 | 照射モード: OTHER / 撮影モード: BRIGHT FIELD |
実験機器 | モデル: Titan Krios / 画像提供: FEI Company |
-画像解析
CTF補正 | ソフトウェア - 名称: CTFFIND (ver. 3.0) |
---|---|
最終 再構成 | 想定した対称性 - 点群: C1 (非対称) / 解像度のタイプ: BY AUTHOR / 解像度: 3.4 Å / 解像度の算出法: FSC 0.143 CUT-OFF / ソフトウェア - 名称: RELION (ver. 1.4) / 使用した粒子像数: 167761 |
初期 角度割当 | タイプ: RANDOM ASSIGNMENT / ソフトウェア - 名称: RELION (ver. 1.4) |
最終 角度割当 | タイプ: RANDOM ASSIGNMENT / ソフトウェア - 名称: RELION (ver. 1.4) |