|Entry||Database: EMDB / ID: 6774|
|Title||Cryo-EM structure of human respiratory complex III (cytochrome bc1 complex)|
|Map data||This map was obtained by sub-region refinement.|
|Sample||Human respiratory complex III:|
|Function / homology||Cytochrome c1 / Cytochrome b-c1 complex subunit 10 / Cytochrome b-c1 complex subunit 8 / Cytochrome b-c1 complex subunit 8 superfamily / Cytochrome b-c1 complex subunit 7 superfamily / Cytochrome b/b6, C-terminal domain superfamily / Cytochrome b / Single alpha-helix domain superfamily / Cytochrome b/b6-like domain superfamily / Ubiquinol-cytochrome C reductase hinge domain ...Cytochrome c1 / Cytochrome b-c1 complex subunit 10 / Cytochrome b-c1 complex subunit 8 / Cytochrome b-c1 complex subunit 8 superfamily / Cytochrome b-c1 complex subunit 7 superfamily / Cytochrome b/b6, C-terminal domain superfamily / Cytochrome b / Single alpha-helix domain superfamily / Cytochrome b/b6-like domain superfamily / Ubiquinol-cytochrome C reductase hinge domain / Cytochrome c1, transmembrane anchor, C-terminal / Rieske [2Fe-2S] iron-sulphur domain / Di-haem cytochrome, transmembrane / Ubiquinol-cytochrome c reductase 8kDa, N-terminal / Rieske iron-sulphur protein / Cytochrome c-like domain superfamily / Peptidase M16, N-terminal / Metalloenzyme, LuxS/M16 peptidase-like / Globular protein, non-globular alpha/beta subunit / Cytochrome b-c1 complex subunit 9 / Peptidase M16, C-terminal / Ubiquinol-cytochrome c reductase, iron-sulphur subunit / Rieske iron-sulphur protein, C-terminal / Cytochrome b/b6, C-terminal / Peptidase M16, zinc-binding site / Cytochrome b/b6, N-terminal / Cytochrome b-c1 complex subunit 7 / Cytochrome b-c1 complex, subunit 6 / Ubiquinol-cytochrome C reductase hinge domain superfamily / Cytochrome b-c1 complex subunit 9 superfamily / Rieske [2Fe-2S] iron-sulphur domain superfamily / Ubiquinol cytochrome reductase transmembrane region / Respiratory electron transport / Mitochondrial protein import / Rieske [2Fe-2S] iron-sulfur domain profile. / Cytochrome b/b6 C-terminal region profile. / Cytochrome b/b6 N-terminal region profile. / Insulinase family, zinc-binding region signature. / Ubiquinol-cytochrome c reductase 8 kDa, N-terminal / Ubiquinol-cytochrome C reductase complex, 6.4kD protein / Ubiquinol-cytochrome C reductase, UQCRX/QCR9 like / Peptidase M16 inactive domain / UcrQ family / Ubiquinol-cytochrome C reductase hinge protein / Insulinase (Peptidase family M16) / Cytochrome b-c1 complex subunit Rieske, transmembrane domain superfamily / Cytochrome b(C-terminal)/b6/petD / Cytochrome b/b6/petB / Rieske [2Fe-2S] domain / Cytochrome b-c1 complex subunit Rieske, transmembrane domain / Cytochrome C1 family / Ubiquinol-cytochrome C reductase complex 14kD subunit / hyperosmotic salinity response / protein processing involved in protein targeting to mitochondrion / subthalamus development / pons development / mitochondrial respiratory chain complex III assembly / response to alkaloid / electron transporter, transferring electrons within CoQH2-cytochrome c reductase complex activity / response to mercury ion / cerebellar Purkinje cell layer development / mitochondrial respiratory chain complex III / mitochondrial respiratory chain / oxidative phosphorylation / ubiquinol—cytochrome-c reductase / ubiquinol-cytochrome-c reductase activity / mitochondrial ATP synthesis coupled proton transport / electron transporter, transferring electrons from CoQH2-cytochrome c reductase complex and cytochrome c oxidase complex activity / integral component of mitochondrial inner membrane / mitochondrial respiratory chain complex IV / thalamus development / pyramidal neuron development / hypothalamus development / response to hormone / response to glucagon / mitochondrial electron transport, ubiquinol to cytochrome c / response to cobalamin / midbrain development / response to copper ion / aerobic respiration / respiratory chain / response to hyperoxia / hippocampus development / protein processing / response to activity / generation of precursor metabolites and energy / 2 iron, 2 sulfur cluster binding / response to calcium ion / animal organ regeneration / metalloendopeptidase activity / mitochondrial inner membrane / response to heat / response to cadmium ion / oxidation-reduction process / response to ethanol / response to hypoxia / electron transfer activity / response to drug / myelin sheath / response to antibiotic|
Function and homology information
|Source||Homo sapiens (human)|
|Method||single particle reconstruction / cryo EM / 3.4 Å resolution|
|Authors||Gu J / Wu M / Yang M|
|Citation||Journal: Cell / Year: 2017|
Title: Architecture of Human Mitochondrial Respiratory Megacomplex IIIIIV.
Authors: Runyu Guo / Shuai Zong / Meng Wu / Jinke Gu / Maojun Yang
Abstract: The respiratory megacomplex represents the highest-order assembly of respiratory chain complexes, and it allows mitochondria to respond to energy-requiring conditions. To understand its architecture, ...The respiratory megacomplex represents the highest-order assembly of respiratory chain complexes, and it allows mitochondria to respond to energy-requiring conditions. To understand its architecture, we examined the human respiratory chain megacomplex-IIIIIV (MCIIIIIV) with 140 subunits and a subset of associated cofactors using cryo-electron microscopy. The MCIIIIIV forms a circular structure with the dimeric CIII located in the center, where it is surrounded by two copies each of CI and CIV. Two cytochrome c (Cyt.c) molecules are positioned to accept electrons on the surface of the c state CIII dimer. Analyses indicate that CII could insert into the gaps between CI and CIV to form a closed ring, which we termed the electron transport chain supercomplex. The structure not only reveals the precise assignment of individual subunits of human CI and CIII, but also enables future in-depth analysis of the electron transport chain as a whole.
|Validation Report||PDB-ID: 5xte|
SummaryFull reportAbout validation report
|Date||Deposition: Jun 18, 2017 / Header (metadata) release: Aug 30, 2017 / Map release: Aug 30, 2017 / Last update: Sep 6, 2017|
|Structure viewer||EM map: |
Downloads & links
|File||emd_6774.map.gz (map file in CCP4 format, 442369 KB)|
|Projections & slices|
Images are generated by Spider.
|Voxel size||X=Y=Z: 1.083 Å|
CCP4 map header:
-Entire Human respiratory complex III
|Entire||Name: Human respiratory complex III / Details: Human cytochrome bc1 complex / Number of components: 1|
|Mass||Experimental: 500 kDa|
-Component #1: protein, Human respiratory complex III
|Protein||Name: Human respiratory complex III / Details: Human cytochrome bc1 complex / Recombinant expression: No|
|Mass||Experimental: 500 kDa|
|Source||Species: Homo sapiens (human)|
|Specimen||Specimen state: particle / Method: cryo EM|
|Sample solution||pH: 7.4|
|Vitrification||Cryogen name: ETHANE|
-Electron microscopy imaging
Model: Titan Krios / Image courtesy: FEI Company
|Imaging||Microscope: FEI TITAN KRIOS|
|Electron gun||Electron source: FIELD EMISSION GUN / Accelerating voltage: 300 kV / Electron dose: 1.25 e/Å2 / Illumination mode: OTHER|
|Lens||Imaging mode: BRIGHT FIELD|
|Specimen Holder||Model: OTHER|
|Camera||Detector: FEI FALCON II (4k x 4k)|
|Processing||Method: single particle reconstruction / Applied symmetry: C1 (asymmetric) / Number of projections: 167761|
|3D reconstruction||Software: RELION / Resolution: 3.4 Å / Resolution method: FSC 0.143 CUT-OFF|
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