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Structure paper

TitleArchitecture of Human Mitochondrial Respiratory Megacomplex IIIIIV.
Journal, issue, pagesCell, Vol. 170, Issue 6, Page 1247-1257.e12, Year 2017
Publish dateSep 7, 2017
AuthorsRunyu Guo / Shuai Zong / Meng Wu / Jinke Gu / Maojun Yang /
PubMed AbstractThe respiratory megacomplex represents the highest-order assembly of respiratory chain complexes, and it allows mitochondria to respond to energy-requiring conditions. To understand its architecture, ...The respiratory megacomplex represents the highest-order assembly of respiratory chain complexes, and it allows mitochondria to respond to energy-requiring conditions. To understand its architecture, we examined the human respiratory chain megacomplex-IIIIIV (MCIIIIIV) with 140 subunits and a subset of associated cofactors using cryo-electron microscopy. The MCIIIIIV forms a circular structure with the dimeric CIII located in the center, where it is surrounded by two copies each of CI and CIV. Two cytochrome c (Cyt.c) molecules are positioned to accept electrons on the surface of the c state CIII dimer. Analyses indicate that CII could insert into the gaps between CI and CIV to form a closed ring, which we termed the electron transport chain supercomplex. The structure not only reveals the precise assignment of individual subunits of human CI and CIII, but also enables future in-depth analysis of the electron transport chain as a whole.
External linksCell / PubMed:28844695
MethodsEM (single particle)
Resolution3.4 - 17.4 Å
Structure data

EMDB-6771, PDB-5xtb:
Cryo-EM structure of human respiratory complex I matrix arm
Method: EM (single particle) / Resolution: 3.4 Å

EMDB-6772, PDB-5xtc:
Cryo-EM structure of human respiratory complex I transmembrane arm
Method: EM (single particle) / Resolution: 3.7 Å

EMDB-6773, PDB-5xtd:
Cryo-EM structure of human respiratory complex I
Method: EM (single particle) / Resolution: 3.7 Å

EMDB-6774, PDB-5xte:
Cryo-EM structure of human respiratory complex III (cytochrome bc1 complex)
Method: EM (single particle) / Resolution: 3.4 Å

EMDB-6775, PDB-5xth:
Cryo-EM structure of human respiratory supercomplex I1III2IV1
Method: EM (single particle) / Resolution: 3.9 Å

EMDB-6776, PDB-5xti:
Cryo-EM architecture of human respiratory chain megacomplex-I2III2IV2
Method: EM (single particle) / Resolution: 17.4 Å

Chemicals

ChemComp-SF4:
IRON/SULFUR CLUSTER / Iron–sulfur cluster

ChemComp-FMN:
FLAVIN MONONUCLEOTIDE / Flavin mononucleotide

ChemComp-8Q1:
S-[2-({N-[(2R)-2-hydroxy-3,3-dimethyl-4-(phosphonooxy)butanoyl]-beta-alanyl}amino)ethyl] dodecanethioate

ChemComp-NDP:
NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE / Nicotinamide adenine dinucleotide phosphate

ChemComp-FES:
FE2/S2 (INORGANIC) CLUSTER / Iron–sulfur cluster

ChemComp-PLX:
(9R,11S)-9-({[(1S)-1-HYDROXYHEXADECYL]OXY}METHYL)-2,2-DIMETHYL-5,7,10-TRIOXA-2LAMBDA~5~-AZA-6LAMBDA~5~-PHOSPHAOCTACOSANE-6,6,11-TRIOL / phospholipid*YM

ChemComp-CDL:
CARDIOLIPIN / phospholipid*YM / Cardiolipin

ChemComp-PEE:
1,2-dioleoyl-sn-glycero-3-phosphoethanolamine / DOPE, phospholipid*YM / Discrete optimized protein energy

ChemComp-HEC:
HEME C / Heme C

ChemComp-HEM:
PROTOPORPHYRIN IX CONTAINING FE / Heme B

ChemComp-CU:
COPPER (II) ION / Copper

ChemComp-MG:
Unknown entry

ChemComp-HEA:
HEME-A / Heme A

ChemComp-ZN:
Unknown entry

Source
  • homo sapiens (human)
  • bos taurus (cattle)
KeywordsOXIDOREDUCTASE/ELECTRON TRANSPORT / Respiratory / OXIDOREDUCTASE-ELECTRON TRANSPORT complex / ELECTRON TRANSPORT / Homo sapiens / Oxidoreductase / Complex / Supercomplex

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