|Entry||Database: EMDB / ID: EMD-6719|
|Title||Cryo-EM structure of mammalian respiratory complex I matrix arm|
|Sample||Mammalian respiratory complex I matix arm|
|Biological species||Sus scrofa (pig)|
|Method||single particle reconstruction / cryo EM / Resolution: 3.38 Å|
|Authors||Gu J / Wu M / Yang M|
|Citation||Journal: Cell / Year: 2016|
Title: Structure of Mammalian Respiratory Supercomplex IIIIIV.
Authors: Meng Wu / Jinke Gu / Runyu Guo / Yushen Huang / Maojun Yang /
Abstract: The mammalian respiratory chain complexes assemble into supercomplexes (SCs) and reside in the inner mitochondrial membrane to transfer electrons and establish the proton gradient for complex V to ...The mammalian respiratory chain complexes assemble into supercomplexes (SCs) and reside in the inner mitochondrial membrane to transfer electrons and establish the proton gradient for complex V to synthesize ATP. The precise arrangement of SCs is largely unknown. Here, we report a 4.0-Å cryo-electron microscopy (cryo-EM) structure of the major SC in porcine heart, the 1.7-MDa SCIIIIIV. The complex III (CIII) dimer and complex IV (CIV) bind at the same side of the L-shaped complex I (CI). Several accessory or supernumerary subunits of CI, such as NDUFA11, NDUFB4, NDUFB8, and NDUFB9, directly contribute to the oligomerization of CI, CIII, and CIV. COX7C and COX7A of CIV attach CIV to the concave surface formed by CIII and the distal end of membrane arm of CI. The structure suggests a possible mechanism by which electrons are transferred from NADH to cytochrome c and provides a platform for future functional dissection of respiration.
|Structure viewer||EM map: |
Downloads & links
|File||Download / File: emd_6719.map.gz / Format: CCP4 / Size: 421.9 MB / Type: IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES)|
|Projections & slices|
Images are generated by Spider.
|Voxel size||X=Y=Z: 1.08 Å|
|Symmetry||Space group: 1|
CCP4 map header:
-Entire Mammalian respiratory complex I matix arm
|Entire||Name: Mammalian respiratory complex I matix arm / Details: Respiratory complex I matix arm from Sus scrofa / Number of components: 1|
-Component #1: protein, Mammalian respiratory complex I matix arm
|Protein||Name: Mammalian respiratory complex I matix arm / Details: Respiratory complex I matix arm from Sus scrofa / Recombinant expression: No|
|Source||Species: Sus scrofa (pig)|
|Specimen||Specimen state: Particle / Method: cryo EM|
|Sample solution||Specimen conc.: 0.2 mg/mL / pH: 7.4|
|Vitrification||Cryogen name: ETHANE / Humidity: 100 %|
-Electron microscopy imaging
Model: Titan Krios / Image courtesy: FEI Company
|Imaging||Microscope: FEI TITAN KRIOS|
|Electron gun||Electron source: FIELD EMISSION GUN / Accelerating voltage: 300 kV / Electron dose: 1.25 e/Å2 / Illumination mode: OTHER|
|Lens||Imaging mode: BRIGHT FIELD|
|Specimen Holder||Model: OTHER|
|Camera||Detector: FEI FALCON II (4k x 4k)|
|Processing||Method: single particle reconstruction / Applied symmetry: C1 (asymmetric) / Number of projections: 161912|
|3D reconstruction||Software: RELION / Resolution: 3.38 Å / Resolution method: FSC 0.143 CUT-OFF|
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