EMDB/PDB/SASBDBから引用されている文献のデータベース

キーワード
構造解析手法	All X線回折 NMR 電子顕微鏡小角散乱中性子線回折線維回折粉末回折赤外分光 EPR FRET 理論モデルハイブリッド
著者・登録者
ジャーナル
IF	>30 >20 >10 >5 all

タイトル	Structure of Mammalian Respiratory Supercomplex IIIIIV.
ジャーナル・号・ページ	Cell, Vol. 167, Issue 6, Page 1598-1609.e10, Year 2016
掲載日	2016年12月1日
著者	Meng Wu / Jinke Gu / Runyu Guo / Yushen Huang / Maojun Yang /
PubMed 要旨	The mammalian respiratory chain complexes assemble into supercomplexes (SCs) and reside in the inner mitochondrial membrane to transfer electrons and establish the proton gradient for complex V to ...The mammalian respiratory chain complexes assemble into supercomplexes (SCs) and reside in the inner mitochondrial membrane to transfer electrons and establish the proton gradient for complex V to synthesize ATP. The precise arrangement of SCs is largely unknown. Here, we report a 4.0-Å cryo-electron microscopy (cryo-EM) structure of the major SC in porcine heart, the 1.7-MDa SCIIIIIV. The complex III (CIII) dimer and complex IV (CIV) bind at the same side of the L-shaped complex I (CI). Several accessory or supernumerary subunits of CI, such as NDUFA11, NDUFB4, NDUFB8, and NDUFB9, directly contribute to the oligomerization of CI, CIII, and CIV. COX7C and COX7A of CIV attach CIV to the concave surface formed by CIII and the distal end of membrane arm of CI. The structure suggests a possible mechanism by which electrons are transferred from NADH to cytochrome c and provides a platform for future functional dissection of respiration.
リンク	Cell / PubMed:27912063
手法	EM (単粒子)
解像度	3.38 - 4.0 Å
構造データ	EMDB-6718: Cryo-EM structure of mammalian respiratory complex III2IV1 by subregion refinement 手法: EM (単粒子) / 解像度: 3.6 Å EMDB-6719: Cryo-EM structure of mammalian respiratory complex I matrix arm 手法: EM (単粒子) / 解像度: 3.38 Å EMDB-6720: Cryo-EM structure of mammalian respiratory complex I 手法: EM (単粒子) / 解像度: 3.62 Å 9539 5gup EMDB-9539, PDB-5gup: Cryo-EM structure of mammalian respiratory supercomplex I1III2IV1 手法: EM (単粒子) / 解像度: 4.0 Å
化合物	ChemComp-SF4: IRON/SULFUR CLUSTER ChemComp-FMN: FLAVIN MONONUCLEOTIDE ChemComp-FES: FE2/S2 (INORGANIC) CLUSTER ChemComp-PLX: (9R,11S)-9-({[(1S)-1-HYDROXYHEXADECYL]OXY}METHYL)-2,2-DIMETHYL-5,7,10-TRIOXA-2LAMBDA~5~-AZA-6LAMBDA~5~-PHOSPHAOCTACOSANE-6,6,11-TRIOL / リン脂質YM ChemComp-NDP: NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE ChemComp-ZMP: S-[2-({N-[(2S)-2-hydroxy-3,3-dimethyl-4-(phosphonooxy)butanoyl]-beta-alanyl}amino)ethyl] tetradecanethioate ChemComp-CDL: CARDIOLIPIN / リン脂質YM ChemComp-PEE: 1,2-dioleoyl-sn-glycero-3-phosphoethanolamine / DOPE, リン脂質YM ChemComp-HEM: PROTOPORPHYRIN IX CONTAINING FE ChemComp-HEC: HEME C ChemComp-HEA: HEME-A ChemComp-CU: COPPER (II) ION ChemComp-MG: Unknown entry ChemComp-ZN*: Unknown entry
由来	sus scrofa (ブタ)
キーワード	ELECTRON TRANSPORT / Cryo-EM / Mammalian / Respiratory / Supercomplex