4I5U
| Crystal structure of a fungal chimeric cellobiohydrolase Cel6A | Descriptor: | 1,2-ETHANEDIOL, ACETATE ION, Chimeric cel6A, ... | Authors: | Arnold, F.H, Wu, I. | Deposit date: | 2012-11-29 | Release date: | 2013-04-03 | Last modified: | 2017-08-09 | Method: | X-RAY DIFFRACTION (1.22 Å) | Cite: | Engineered thermostable fungal Cel6A and Cel7A cellobiohydrolases hydrolyze cellulose efficiently at elevated temperatures. Biotechnol.Bioeng., 110, 2013
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4I5R
| Crystal structure of a fungal chimeric cellobiohydrolase Cel6A | Descriptor: | 1,2-ETHANEDIOL, ACETATE ION, Chimeric cel6A, ... | Authors: | Arnold, F.H, Wu, I. | Deposit date: | 2012-11-28 | Release date: | 2013-04-03 | Last modified: | 2017-08-09 | Method: | X-RAY DIFFRACTION (1.5 Å) | Cite: | Engineered thermostable fungal Cel6A and Cel7A cellobiohydrolases hydrolyze cellulose efficiently at elevated temperatures. Biotechnol.Bioeng., 110, 2013
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4WG2
| P411BM3-CIS T438S I263F regioselective C-H amination catalyst | Descriptor: | Bifunctional P-450/NADPH-P450 reductase, PROTOPORPHYRIN IX CONTAINING FE, SULFATE ION | Authors: | Hyster, T.K, Farwell, C.C, Buller, A.R, McIntosh, J.A, Arnold, F.H. | Deposit date: | 2014-09-17 | Release date: | 2014-11-05 | Last modified: | 2023-12-27 | Method: | X-RAY DIFFRACTION (2.66 Å) | Cite: | Enzyme-controlled nitrogen-atom transfer enables regiodivergent C-h amination. J.Am.Chem.Soc., 136, 2014
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4XDZ
| Holo structure of ketol-acid reductoisomerase from Ignisphaera aggregans | Descriptor: | 4-(2-HYDROXYETHYL)-1-PIPERAZINE ETHANESULFONIC ACID, GLYCEROL, Ketol-acid reductoisomerase, ... | Authors: | Cahn, J.K.B, Brinkmann-Chen, S, Arnold, F.H. | Deposit date: | 2014-12-20 | Release date: | 2015-04-22 | Last modified: | 2023-09-27 | Method: | X-RAY DIFFRACTION (1.15 Å) | Cite: | Cofactor specificity motifs and the induced fit mechanism in class I ketol-acid reductoisomerases. Biochem.J., 468, 2015
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4XDY
| Structure of NADH-preferring ketol-acid reductoisomerase from an uncultured archean | Descriptor: | 1,4-DIHYDRONICOTINAMIDE ADENINE DINUCLEOTIDE, GLYCEROL, Ketol-acid reductoisomerase, ... | Authors: | Cahn, J.K.B, Brinkmann-Chen, S, Arnold, F.H. | Deposit date: | 2014-12-20 | Release date: | 2015-04-22 | Last modified: | 2023-09-27 | Method: | X-RAY DIFFRACTION (1.535 Å) | Cite: | Cofactor specificity motifs and the induced fit mechanism in class I ketol-acid reductoisomerases. Biochem.J., 468, 2015
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4XEH
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8EGZ
| Engineered tyrosine synthase (TmTyrS1) derived from T. maritima TrpB with Ser bound as the amino-acrylate intermediate | Descriptor: | 1,2-ETHANEDIOL, 2-{[(E)-{3-hydroxy-2-methyl-5-[(phosphonooxy)methyl]pyridin-4-yl}methylidene]amino}prop-2-enoic acid, POTASSIUM ION, ... | Authors: | Porter, N.J, Almhjell, P.J, Arnold, F.H. | Deposit date: | 2022-09-13 | Release date: | 2023-10-04 | Last modified: | 2024-08-14 | Method: | X-RAY DIFFRACTION (1.9 Å) | Cite: | The beta-subunit of tryptophan synthase is a latent tyrosine synthase. Nat.Chem.Biol., 20, 2024
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8EH0
| Engineered tyrosine synthase (TmTyrS1) derived from T. maritima TrpB with Ser bound as the amino-acrylate intermediate and complexed with quinoline N-oxide | Descriptor: | 1,2-ETHANEDIOL, 1-oxo-1lambda~5~-quinoline, 2-{[(E)-{3-hydroxy-2-methyl-5-[(phosphonooxy)methyl]pyridin-4-yl}methylidene]amino}prop-2-enoic acid, ... | Authors: | Porter, N.J, Almhjell, P.J, Arnold, F.H. | Deposit date: | 2022-09-13 | Release date: | 2023-10-04 | Last modified: | 2024-08-14 | Method: | X-RAY DIFFRACTION (1.7 Å) | Cite: | The beta-subunit of tryptophan synthase is a latent tyrosine synthase. Nat.Chem.Biol., 20, 2024
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8EH1
| Engineered tyrosine synthase (TmTyrS1) derived from T. maritima TrpB with Ser bound as the amino-acrylate intermediate and complexed with 4-hydroxyquinoline | Descriptor: | 2-{[(E)-{3-hydroxy-2-methyl-5-[(phosphonooxy)methyl]pyridin-4-yl}methylidene]amino}prop-2-enoic acid, Engineered tyrosine synthase (TmTyrS1), POTASSIUM ION, ... | Authors: | Porter, N.J, Almhjell, P.J, Arnold, F.H. | Deposit date: | 2022-09-13 | Release date: | 2023-10-04 | Last modified: | 2024-08-14 | Method: | X-RAY DIFFRACTION (2 Å) | Cite: | The beta-subunit of tryptophan synthase is a latent tyrosine synthase. Nat.Chem.Biol., 20, 2024
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8EGY
| Engineered holo tyrosine synthase (TmTyrS1) derived from T. maritima TrpB | Descriptor: | 1,2-ETHANEDIOL, PHOSPHATE ION, POTASSIUM ION, ... | Authors: | Porter, N.J, Almhjell, P.J, Arnold, F.H. | Deposit date: | 2022-09-13 | Release date: | 2023-10-04 | Last modified: | 2024-08-14 | Method: | X-RAY DIFFRACTION (2.05 Å) | Cite: | The beta-subunit of tryptophan synthase is a latent tyrosine synthase. Nat.Chem.Biol., 20, 2024
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8DSG
| P411-PFA carbene transferase | Descriptor: | 1,2-ETHANEDIOL, 2-[BIS-(2-HYDROXY-ETHYL)-AMINO]-2-HYDROXYMETHYL-PROPANE-1,3-DIOL, Cytochrome P450-BM3 variant P411-PFA, ... | Authors: | Maggiolo, A.O, Porter, N.J, Zhang, J, Arnold, F.H. | Deposit date: | 2022-07-22 | Release date: | 2023-03-08 | Last modified: | 2024-05-01 | Method: | X-RAY DIFFRACTION (1.87 Å) | Cite: | Chemodivergent C(sp 3 )-H and C(sp 2 )-H Cyanomethylation Using Engineered Carbene Transferases. Nat Catal, 6, 2023
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6WK3
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5DW3
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5DVZ
| Holo TrpB from Pyrococcus furiosus | Descriptor: | PHOSPHATE ION, SODIUM ION, Tryptophan synthase beta chain 1 | Authors: | Buller, A.R, Arnold, F.H. | Deposit date: | 2015-09-21 | Release date: | 2016-02-03 | Last modified: | 2019-12-25 | Method: | X-RAY DIFFRACTION (1.69 Å) | Cite: | Directed evolution of the tryptophan synthase beta-subunit for stand-alone function recapitulates allosteric activation. Proc.Natl.Acad.Sci.USA, 112, 2015
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5IXJ
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5E0K
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5E4R
| Crystal structure of domain-duplicated synthetic class II ketol-acid reductoisomerase 2Ia_KARI-DD | Descriptor: | 1,2-ETHANEDIOL, GLYCEROL, Ketol-acid reductoisomerase, ... | Authors: | Cahn, J.K.B, Brinkmann-Chen, S, Buller, A.R, Arnold, F.H. | Deposit date: | 2015-10-07 | Release date: | 2015-12-23 | Last modified: | 2023-09-27 | Method: | X-RAY DIFFRACTION (1.94 Å) | Cite: | Artificial domain duplication replicates evolutionary history of ketol-acid reductoisomerases. Protein Sci., 25, 2016
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8VHH
| Engineered holo tryptophan synthase (Tm9D8*) derived from T. maritima TrpB | Descriptor: | PHOSPHATE ION, POTASSIUM ION, PYRIDOXAL-5'-PHOSPHATE, ... | Authors: | Porter, N.J, Johnston, K.E, Almhjell, P.J, Arnold, F.H. | Deposit date: | 2024-01-02 | Release date: | 2024-08-14 | Method: | X-RAY DIFFRACTION (2.15 Å) | Cite: | A combinatorially complete epistatic fitness landscape in an enzyme active site. Proc.Natl.Acad.Sci.USA, 121, 2024
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4KQX
| Mutant Slackia exigua KARI DDV in complex with NAD and an inhibitor | Descriptor: | HISTIDINE, Ketol-acid reductoisomerase, MAGNESIUM ION, ... | Authors: | Brinkmann-Chen, S, Flock, T, Cahn, J.K.B, Snow, C.D, Brustad, E.M, Mcintosh, J.A, Meinhold, P, Zhang, L, Arnold, F.H. | Deposit date: | 2013-05-15 | Release date: | 2013-06-26 | Last modified: | 2023-09-20 | Method: | X-RAY DIFFRACTION (1.8 Å) | Cite: | General approach to reversing ketol-acid reductoisomerase cofactor dependence from NADPH to NADH. Proc.Natl.Acad.Sci.USA, 110, 2013
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4KQW
| The structure of the Slackia exigua KARI in complex with NADP | Descriptor: | Ketol-acid reductoisomerase, L(+)-TARTARIC ACID, MAGNESIUM ION, ... | Authors: | Brinkmann-Chen, S, Flock, T, Cahn, J.K.B, Snow, C.D, Brustad, E.M, Mcintosh, J.A, Meinhold, P, Zhang, L, Arnold, F.H. | Deposit date: | 2013-05-15 | Release date: | 2013-06-26 | Last modified: | 2023-09-20 | Method: | X-RAY DIFFRACTION (1.39 Å) | Cite: | General approach to reversing ketol-acid reductoisomerase cofactor dependence from NADPH to NADH. Proc.Natl.Acad.Sci.USA, 110, 2013
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3QI8
| Evolved variant of cytochrome P450 (BM3, CYP102A1) | Descriptor: | Evolved Cytochrome P450 variant (22A3), PROTOPORPHYRIN IX CONTAINING FE | Authors: | Rentmeister, A, Brown, T.R, Snow, C.D, Carbone, M.N, Arnold, F.H. | Deposit date: | 2011-01-26 | Release date: | 2011-05-04 | Last modified: | 2023-09-13 | Method: | X-RAY DIFFRACTION (3.2 Å) | Cite: | Engineered Bacterial Mimics of Human Drug Metabolizing Enzyme CYP2C9 Chemcatchem, 2011
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5BR4
| E. coli lactaldehyde reductase (FucO) M185C mutant | Descriptor: | CHLORIDE ION, GLYCEROL, Lactaldehyde reductase, ... | Authors: | Cahn, J.K.B, Brinkmann-Chen, S, Arnold, F.H. | Deposit date: | 2015-05-29 | Release date: | 2015-12-23 | Last modified: | 2023-09-27 | Method: | X-RAY DIFFRACTION (0.91 Å) | Cite: | Mutations in adenine-binding pockets enhance catalytic properties of NAD(P)H-dependent enzymes. Protein Eng.Des.Sel., 29, 2016
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4XIY
| Crystal structure of ketol-acid reductoisomerase from Azotobacter | Descriptor: | DI(HYDROXYETHYL)ETHER, FE (III) ION, Ketol-acid reductoisomerase, ... | Authors: | Spatzal, T, Cahn, J.K.B, Wiig, J.A, Einsle, O, Hu, Y, Ribbe, M.W, Arnold, F.H. | Deposit date: | 2015-01-07 | Release date: | 2015-04-22 | Last modified: | 2023-09-27 | Method: | X-RAY DIFFRACTION (2.5 Å) | Cite: | Cofactor specificity motifs and the induced fit mechanism in class I ketol-acid reductoisomerases. Biochem.J., 468, 2015
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5D3U
| Crystal structure of the 5-selective H176F mutant of Cytochrome TxtE | Descriptor: | CHLORIDE ION, GLYCEROL, P450-like protein, ... | Authors: | Cahn, J.K.B, Dodani, S.C, Arnold, F.H. | Deposit date: | 2015-08-06 | Release date: | 2016-06-22 | Last modified: | 2023-09-27 | Method: | X-RAY DIFFRACTION (1.45 Å) | Cite: | Discovery of a regioselectivity switch in nitrating P450s guided by molecular dynamics simulations and Markov models. Nat.Chem., 8, 2016
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3QR3
| Crystal Structure of Cel5A (EG2) from Hypocrea jecorina (Trichoderma reesei) | Descriptor: | Endoglucanase EG-II, MAGNESIUM ION, SULFATE ION | Authors: | Lee, T.M, Farrow, M.F, Kaiser, J.T, Arnold, F.H, Mayo, S.L. | Deposit date: | 2011-02-16 | Release date: | 2011-11-02 | Last modified: | 2024-10-16 | Method: | X-RAY DIFFRACTION (2.05 Å) | Cite: | A structural study of Hypocrea jecorina Cel5A. Protein Sci., 20, 2011
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