8EGZ

Engineered tyrosine synthase (TmTyrS1) derived from T. maritima TrpB with Ser bound as the amino-acrylate intermediate

Summary for 8EGZ

• Entry DOI: 10.2210/pdb8egz/pdb
• Descriptor: Tryptophan synthase beta chain 1, 2-{[(E)-{3-hydroxy-2-methyl-5-[(phosphonooxy)methyl]pyridin-4-yl}methylidene]amino}prop-2-enoic acid, 1,2-ETHANEDIOL, ... (5 entities in total)
• Functional Keywords: tyrosine synthase, engineered enzyme, noncanonical amino acid synthase, biosynthetic protein
• Biological source: Thermotoga maritima
• Total number of polymer chains: 2
• Total formula weight: 88486.57

Authors

Porter, N.J.,Almhjell, P.J.,Arnold, F.H. (deposition date: 2022-09-13, release date: 2023-10-04, Last modification date: 2024-07-03)

• Primary citation: Almhjell, P.J.,Johnston, K.E.,Porter, N.J.,Kennemur, J.L.,Bhethanabotla, V.C.,Ducharme, J.,Arnold, F.H.; The beta-subunit of tryptophan synthase is a latent tyrosine synthase.; Nat.Chem.Biol., 2024; PubMed: 38744987; DOI: 10.1038/s41589-024-01619-z

Experimental method

X-RAY DIFFRACTION (1.9 Å)