8EH0

Engineered tyrosine synthase (TmTyrS1) derived from T. maritima TrpB with Ser bound as the amino-acrylate intermediate and complexed with quinoline N-oxide

Summary for 8EH0

Entry DOI	10.2210/pdb8eh0/pdb
Descriptor	Tryptophan synthase beta chain 1, 2-{[(E)-{3-hydroxy-2-methyl-5-[(phosphonooxy)methyl]pyridin-4-yl}methylidene]amino}prop-2-enoic acid, 1,2-ETHANEDIOL, ... (6 entities in total)
Functional Keywords	tyrosine synthase, engineered enzyme, noncanonical amino acid synthase, biosynthetic protein
Biological source	Thermotoga maritima
Total number of polymer chains	2
Total formula weight	88652.75
Authors	Porter, N.J.,Almhjell, P.J.,Arnold, F.H. (deposition date: 2022-09-13, release date: 2023-10-04, Last modification date: 2024-07-03)
Primary citation	Almhjell, P.J.,Johnston, K.E.,Porter, N.J.,Kennemur, J.L.,Bhethanabotla, V.C.,Ducharme, J.,Arnold, F.H. The beta-subunit of tryptophan synthase is a latent tyrosine synthase. Nat.Chem.Biol., 2024 Cited by PubMed: 38744987 DOI: 10.1038/s41589-024-01619-z PDB entries with the same primary citation
Experimental method	X-RAY DIFFRACTION (1.7 Å)

Structure validation