5FQI
| W229D and F290W mutant of the last common ancestor of Gram-negative bacteria (GNCA4) beta-lactamase class A | Descriptor: | 1,2-ETHANEDIOL, 2-(2-METHOXYETHOXY)ETHANOL, DI(HYDROXYETHYL)ETHER, ... | Authors: | Gavira, J.A, Risso, V.A, Martinez-Rodriguez, S, Sanchez-Ruiz, J.M. | Deposit date: | 2015-12-11 | Release date: | 2016-12-21 | Last modified: | 2024-01-10 | Method: | X-RAY DIFFRACTION (1.4 Å) | Cite: | De novo active sites for resurrected Precambrian enzymes. Nat Commun, 8, 2017
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5FQK
| W229D and F290W mutant of the last common ancestor of Gram-negative bacteria (GNCA4) beta-lactamase class A bound to 5(6)-nitrobenzotriazole (TS-analog) | Descriptor: | 6-NITROBENZOTRIAZOLE, GNCA4 LACTAMASE W229D AND F290W | Authors: | Gavira, J.A, Risso, V.A, Martinez-Rodriguez, S, Sanchez-Ruiz, J.M. | Deposit date: | 2015-12-11 | Release date: | 2016-12-21 | Last modified: | 2024-01-10 | Method: | X-RAY DIFFRACTION (1.767 Å) | Cite: | De novo active sites for resurrected Precambrian enzymes. Nat Commun, 8, 2017
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7OOL
| Crystal structure of a Candidatus photodesmus katoptron thioredoxin chimera containing an ancestral loop | Descriptor: | 1,2-ETHANEDIOL, DI(HYDROXYETHYL)ETHER, GLYCEROL, ... | Authors: | Gavira, J.A, Ibarra-Molero, B, Gamiz-Arco, G, Risso, V, Sanchez-Ruiz, J.M. | Deposit date: | 2021-05-28 | Release date: | 2021-11-10 | Last modified: | 2024-01-31 | Method: | X-RAY DIFFRACTION (2.85 Å) | Cite: | Combining Ancestral Reconstruction with Folding-Landscape Simulations to Engineer Heterologous Protein Expression. J.Mol.Biol., 433, 2021
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2FD3
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2FCH
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5FQQ
| Last common ancestor of Gram-negative bacteria (GNCA4) beta-lactamase class A | Descriptor: | 2-(2-METHOXYETHOXY)ETHANOL, DI(HYDROXYETHYL)ETHER, GNCA4 LACTAMASE | Authors: | Gavira, J.A, Martinez-Rodriguez, S, Risso, V.A, Sanchez-Ruiz, J.M. | Deposit date: | 2015-12-14 | Release date: | 2016-12-28 | Last modified: | 2024-01-10 | Method: | X-RAY DIFFRACTION (2.12 Å) | Cite: | De novo active sites for resurrected Precambrian enzymes. Nat Commun, 8, 2017
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2K6R
| Protein folding on a highly rugged landscape: Experimental observation of glassy dynamics and structural frustration | Descriptor: | Full Sequence Design 1 Synthetic Superstable | Authors: | Sadqi, M, de Alba, E, Perez-Jimenez, R, Sanchez-Ruiz, J.M, Munoz, V. | Deposit date: | 2008-07-18 | Release date: | 2009-06-16 | Last modified: | 2016-06-08 | Method: | SOLUTION NMR | Cite: | A designed protein as experimental model of primordial folding Proc.Natl.Acad.Sci.USA, 106, 2009
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4C6Y
| Ancestral PNCA (last common ancestors of Gram-positive and Gram- negative bacteria) beta-lactamase class A | Descriptor: | ACETATE ION, BETA-LACTAMASE, CHLORIDE ION, ... | Authors: | Gavira, J.A, Risso, V.A, Sanchez-Ruiz, J.M. | Deposit date: | 2013-09-19 | Release date: | 2014-04-16 | Last modified: | 2023-12-20 | Method: | X-RAY DIFFRACTION (1.799 Å) | Cite: | Phenotypic Comparisons of Consensus Variants Versus Laboratory Resurrections of Precambrian Proteins. Proteins, 82, 2014
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4C75
| Consensus (ALL-CON) beta-lactamase class A | Descriptor: | ACETATE ION, BETA-LACTAMASE, DI(HYDROXYETHYL)ETHER, ... | Authors: | Gavira, J.A, Risso, V.A, Sanchez-Ruiz, J.M. | Deposit date: | 2013-09-19 | Release date: | 2014-04-16 | Last modified: | 2023-12-20 | Method: | X-RAY DIFFRACTION (2.2 Å) | Cite: | Phenotypic Comparisons of Consensus Variants Versus Laboratory Resurrections of Precambrian Proteins. Proteins, 82, 2014
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3ZHC
| Structure of the phytase from Citrobacter braakii at 2.3 angstrom resolution. | Descriptor: | CHLORIDE ION, FORMIC ACID, PHYTASE | Authors: | Wilson, K.S, Ariza, A, Sanchez-Romero, I, Skjot, M, Vind, J, DeMaria, L, Skov, L.K, Sanchez-Ruiz, J.M. | Deposit date: | 2012-12-20 | Release date: | 2013-08-28 | Last modified: | 2017-08-09 | Method: | X-RAY DIFFRACTION (2.3 Å) | Cite: | Mechanism of Protein Kinetic Stabilization by Engineered Disulfide Crosslinks Plos One, 8, 2013
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4BA7
| Crystal Structure of Ancestral Thioredoxin Relative to Last Bacteria Common Ancestor (LBCA) from the Precambrian Period | Descriptor: | 1,2-ETHANEDIOL, LBCA THIOREDOXIN | Authors: | Gavira, J.A, Ingles-Prieto, A, Ibarra-Molero, B, Sanchez-Ruiz, J.M. | Deposit date: | 2012-09-12 | Release date: | 2013-08-21 | Last modified: | 2023-12-20 | Method: | X-RAY DIFFRACTION (2.45 Å) | Cite: | Conservation of Protein Structure Over Four Billion Years Structure, 21, 2013
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3ZIV
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4B88
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3ZDJ
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6QIZ
| CI-2, conformation 2 | Descriptor: | Subtilisin-chymotrypsin inhibitor-2A | Authors: | Romero, A, Ruiz, F.M. | Deposit date: | 2019-01-21 | Release date: | 2019-12-25 | Last modified: | 2024-01-24 | Method: | X-RAY DIFFRACTION (1.65 Å) | Cite: | Engineering protein assemblies with allosteric control via monomer fold-switching. Nat Commun, 10, 2019
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6QIY
| CI-2, conformation 1 | Descriptor: | Subtilisin-chymotrypsin inhibitor-2A | Authors: | Romero, A, Ruiz, F.M. | Deposit date: | 2019-01-21 | Release date: | 2019-12-25 | Last modified: | 2024-01-24 | Method: | X-RAY DIFFRACTION (1.5 Å) | Cite: | Engineering protein assemblies with allosteric control via monomer fold-switching. Nat Commun, 10, 2019
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2YJ7
| Crystal structure of a hyperstable protein from the Precambrian period | Descriptor: | LPBCA THIOREDOXIN, SODIUM ION | Authors: | Gavira, J.A, Ingles, A, Ibarra, B, Garcia-Ruiz, J.M. | Deposit date: | 2011-05-19 | Release date: | 2012-05-30 | Last modified: | 2023-12-20 | Method: | X-RAY DIFFRACTION (1.65 Å) | Cite: | Conservation of Protein Structure Over Four Billion Years Structure, 21, 2013
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4HHP
| Crystal structure of triosephosphate isomerase from trypanosoma cruzi, mutant e105d | Descriptor: | GLYCEROL, SULFATE ION, Triosephosphate isomerase, ... | Authors: | Hernandez-Santoyo, A, Aguirre-Fuentes, Y, Torres-Larios, A, Gomez-Puyou, A, De Gomez-Puyou, M.T. | Deposit date: | 2012-10-10 | Release date: | 2013-10-16 | Last modified: | 2024-02-28 | Method: | X-RAY DIFFRACTION (1.5 Å) | Cite: | Different contribution of conserved amino acids to the global properties of triosephosphate isomerases. Proteins, 82, 2014
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4JEQ
| Different Contribution of Conserved Amino Acids to the Global Properties of Homologous Enzymes | Descriptor: | DI(HYDROXYETHYL)ETHER, SULFATE ION, TRIOSEPHOSPHATE ISOMERASE, ... | Authors: | Hernandez-Santoyo, A, Aguirre-Fuentes, Y, Torres-Larios, A, Gomez-Puyou, A, De Gomez-Puyou, M.T. | Deposit date: | 2013-02-27 | Release date: | 2013-10-02 | Last modified: | 2023-09-20 | Method: | X-RAY DIFFRACTION (2.303 Å) | Cite: | Different contribution of conserved amino acids to the global properties of triosephosphate isomerases. Proteins, 82, 2014
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1R4Y
| SOLUTION STRUCTURE OF THE DELETION MUTANT DELTA(7-22) OF THE CYTOTOXIC RIBONUCLEASE ALPHA-SARCIN | Descriptor: | Ribonuclease alpha-sarcin | Authors: | Garcia-Mayoral, M.F, Garcia-Ortega, L, Lillo, M.P, Santoro, J, Martinez Del Pozo, A, Gavilanes, J.G, Rico, M, Bruix, M. | Deposit date: | 2003-10-09 | Release date: | 2004-04-06 | Last modified: | 2021-10-27 | Method: | SOLUTION NMR | Cite: | NMR structure of the noncytotoxic {alpha}-sarcin mutant {Delta}(7-22): The importance of the native conformation of peripheral loops for activity. Protein Sci., 13, 2004
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4CBS
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4CBR
| X-ray structure of the more stable human AGXT triple mutant (AGXT_HEM) | Descriptor: | GLYCEROL, PYRIDOXAL-5'-PHOSPHATE, SERINE--PYRUVATE AMINOTRANSFERASE | Authors: | Yunta, C, Albert, A. | Deposit date: | 2013-10-16 | Release date: | 2014-07-09 | Last modified: | 2023-12-20 | Method: | X-RAY DIFFRACTION (2.3 Å) | Cite: | The Consensus-Based Approach for Gene/Enzyme Replacement Therapies and Crystallization Strategies: The Case of Human Alanine:Glyoxylate Aminotransferase. Biochem.J., 462, 2014
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