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7PFP

Full-length cryo-EM structure of the native human uromodulin (UMOD)/Tamm-Horsfall protein (THP) filament

Summary for 7PFP
Entry DOI10.2210/pdb7pfp/pdb
Related4WRN 6TQK 6TQL 7P6R 7P6S 7P6T
EMDB information10553 10554 13378
DescriptorUromodulin, 2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-[alpha-L-fucopyranose-(1-6)]2-acetamido-2-deoxy-beta-D-glucopyranose, beta-D-mannopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose, ... (8 entities in total)
Functional Keywordsegf domain, decoy module, beta-hairpin, d10c domain, zp module, zp domain, zp-n domain, zp-c domain, interdomain linker, extracellular matrix, glycoprotein, n-glycan, structural protein, protein filament, protein polymerization, antimicrobial protein
Biological sourceHomo sapiens (Human)
Total number of polymer chains3
Total formula weight224801.05
Authors
Jovine, L.,Xu, C.,Stsiapanava, A.,Carroni, M.,Tunyasuvunakool, K.,Jumper, J.,Wu, B. (deposition date: 2021-08-11, release date: 2022-03-16, Last modification date: 2024-10-16)
Primary citationStsiapanava, A.,Xu, C.,Nishio, S.,Han, L.,Yamakawa, N.,Carroni, M.,Tunyasuvunakool, K.,Jumper, J.,de Sanctis, D.,Wu, B.,Jovine, L.
Structure of the decoy module of human glycoprotein 2 and uromodulin and its interaction with bacterial adhesin FimH.
Nat.Struct.Mol.Biol., 29:190-193, 2022
Cited by
PubMed Abstract: Glycoprotein 2 (GP2) and uromodulin (UMOD) filaments protect against gastrointestinal and urinary tract infections by acting as decoys for bacterial fimbrial lectin FimH. By combining AlphaFold2 predictions with X-ray crystallography and cryo-EM, we show that these proteins contain a bipartite decoy module whose new fold presents the high-mannose glycan recognized by FimH. The structure rationalizes UMOD mutations associated with kidney diseases and visualizes a key epitope implicated in cast nephropathy.
PubMed: 35273390
DOI: 10.1038/s41594-022-00729-3
PDB entries with the same primary citation
Experimental method
ELECTRON MICROSCOPY (6.1 Å)
Structure validation

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