7P6T
Crystal structure of the FimH-binding decoy module of human glycoprotein 2 (GP2) (crystal form III)
Summary for 7P6T
| Entry DOI | 10.2210/pdb7p6t/pdb |
| Related | 7P6R 7P6S |
| Descriptor | Isoform Alpha of Pancreatic secretory granule membrane major glycoprotein GP2, 2-acetamido-2-deoxy-beta-D-glucopyranose, 2-ethyl-2-(hydroxymethyl)propane-1,3-diol, ... (4 entities in total) |
| Functional Keywords | extracellular matrix, glycoprotein, n-glycan, structural protein, decoy module, d10c domain, d8c sequence, pancreas, intestine, mucosal immunity, fimh, alphafold, antimicrobial protein |
| Biological source | Homo sapiens (Human) |
| Total number of polymer chains | 1 |
| Total formula weight | 19245.35 |
| Authors | Stsiapanava, A.,Tunyasuvunakool, K.,Jumper, J.,de Sanctis, D.,Jovine, L. (deposition date: 2021-07-17, release date: 2022-03-16, Last modification date: 2024-10-09) |
| Primary citation | Stsiapanava, A.,Xu, C.,Nishio, S.,Han, L.,Yamakawa, N.,Carroni, M.,Tunyasuvunakool, K.,Jumper, J.,de Sanctis, D.,Wu, B.,Jovine, L. Structure of the decoy module of human glycoprotein 2 and uromodulin and its interaction with bacterial adhesin FimH. Nat.Struct.Mol.Biol., 29:190-193, 2022 Cited by PubMed Abstract: Glycoprotein 2 (GP2) and uromodulin (UMOD) filaments protect against gastrointestinal and urinary tract infections by acting as decoys for bacterial fimbrial lectin FimH. By combining AlphaFold2 predictions with X-ray crystallography and cryo-EM, we show that these proteins contain a bipartite decoy module whose new fold presents the high-mannose glycan recognized by FimH. The structure rationalizes UMOD mutations associated with kidney diseases and visualizes a key epitope implicated in cast nephropathy. PubMed: 35273390DOI: 10.1038/s41594-022-00729-3 PDB entries with the same primary citation |
| Experimental method | X-RAY DIFFRACTION (1.4 Å) |
Structure validation
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