6J64

Crystal structure of human HINT1 mutant complexing with AP4A

6J64 の概要

• エントリーDOI: 10.2210/pdb6j64/pdb
• 関連するPDBエントリー: 5ED3 5ED6 6J53 6J58 6J5S 6J5Z
• 分子名称: Histidine triad nucleotide-binding protein 1, 2-AMINOETHANESULFONIC ACID, BIS(ADENOSINE)-5'-TETRAPHOSPHATE, ... (4 entities in total)
• 機能のキーワード: hydrolase, nucleotide binding, regulation of transcription, signal transduction
• 由来する生物種: Homo sapiens (Human)
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 28875.73

構造登録者

Wang, J.,Fang, P.,Guo, M. (登録日: 2019-01-14, 公開日: 2019-09-25, 最終更新日: 2023-11-22)

主引用文献

Yu, J.,Liu, Z.,Liang, Y.,Luo, F.,Zhang, J.,Tian, C.,Motzik, A.,Zheng, M.,Kang, J.,Zhong, G.,Liu, C.,Fang, P.,Guo, M.,Razin, E.,Wang, J.
Second messenger Ap4A polymerizes target protein HINT1 to transduce signals in Fc epsilon RI-activated mast cells.
Nat Commun, 10:4664-4664, 2019

PubMed Abstract: Signal transduction systems enable organisms to monitor their external environments and accordingly adjust the cellular processes. In mast cells, the second messenger ApA binds to the histidine triad nucleotide-binding protein 1 (HINT1), disrupts its interaction with the microphthalmia-associated transcription factor (MITF), and eventually activates the transcription of genes downstream of MITF in response to immunostimulation. How the HINT1 protein recognizes and is regulated by ApA remain unclear. Here, using eight crystal structures, biochemical experiments, negative stain electron microscopy, and cellular experiments, we report that ApA specifically polymerizes HINT1 in solution and in activated rat basophilic leukemia cells. The polymerization interface overlaps with the area on HINT1 for MITF interaction, suggesting a possible competitive mechanism to release MITF for transcriptional activation. The mechanism depends precisely on the length of the phosphodiester linkage of ApA. These results highlight a direct polymerization signaling mechanism by the second messenger.

PubMed: 31604935
DOI: 10.1038/s41467-019-12710-8

実験手法

X-RAY DIFFRACTION (0.95 Å)