4QGZ

X-ray structure of the adduct formed between hen egg white lysozyme and trans-dimethylamine methylamine dichlorido platinum(II)

4QGZ の概要

• エントリーDOI: 10.2210/pdb4qgz/pdb
• 関連するPDBエントリー: 2I6Z 4J1A 4J1B 4LFK 4LFP 4LGK 4MR1 4NY5 4QH3
• 分子名称: Lysozyme C, bis(chloranyl)-(dimethylamino)-(methylamino)platinum (3 entities in total)
• 機能のキーワード: lysozyme fold, hydrolase, o-glycosyl hydrolase
• 由来する生物種: Gallus gallus (bantam,chickens)
• 細胞内の位置: Secreted: P00698
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 15011.38

構造登録者

Russo Krauss, I.,Merlino, A. (登録日: 2014-05-26, 公開日: 2014-07-30, 最終更新日: 2014-08-20)

主引用文献

Messori, L.,Marzo, T.,Michelucci, E.,Russo Krauss, I.,Navarro-Ranninger, C.,Quiroga, A.G.,Merlino, A.
Interactions between Anticancer trans-Platinum Compounds and Proteins: Crystal Structures and ESI-MS Spectra of Two Protein Adducts of trans-(Dimethylamino)(methylamino)dichloridoplatinum(II).
Inorg.Chem., 53:7806-7808, 2014

PubMed Abstract: The adducts formed between trans-(dimethylamino)(methylamino)dichloridoplatinum(II), [t-PtCl2(dma)(ma)], and two model proteins, i.e., hen egg white lysozyme and bovine pancreatic ribonuclease, were independently characterized by X-ray crystallography and electrospray ionization mass spectrometry. In these adducts, the Pt(II) center, upon chloride release, coordinates either to histidine or aspartic acid residues while both alkylamino ligands remain bound to the metal. Comparison with the cisplatin derivatives of the same proteins highlights for [t-PtCl2(dma)(ma)] a kind of biomolecular metalation remarkably different from that of cisplatin.

PubMed: 25025479
DOI: 10.1021/ic5012583

実験手法

X-RAY DIFFRACTION (2.51 Å)