Structure of Thermus thermophilus Argonaute bound to guide DNA 19-mer and target DNA in the presence of MN2+

Summary for 4NCA

Related4N41 4N47 4N76 4NCB
DescriptorArgonaute, 5'-D(P*TP*GP*AP*GP*GP*TP*AP*GP*TP*AP*GP*GP*TP*TP*GP*TP*AP*TP*AP*GP*T)-3', 5'-D(P*TP*AP*CP*TP*AP*CP*CP*TP*CP*G)-3', ... (7 entities in total)
Functional Keywordsargonaute, rna interference, dna interference, nuclear protein-dna complex, nuclear protein/dna
Biological sourceThermus thermophilus
Total number of polymer chains8
Total molecular weight176908.6
Sheng, G.,Zhao, H.,Wang, J.,Rao, Y.,Wang, Y. (deposition date: 2013-10-24, release date: 2014-01-15, Last modification date: 2017-11-15)
Primary citation
Sheng, G.,Zhao, H.,Wang, J.,Rao, Y.,Tian, W.,Swarts, D.C.,van der Oost, J.,Patel, D.J.,Wang, Y.
Structure-based cleavage mechanism of Thermus thermophilus Argonaute DNA guide strand-mediated DNA target cleavage.
Proc.Natl.Acad.Sci.USA, 111:652-657, 2014
PubMed: 24374628 (PDB entries with the same primary citation)
DOI: 10.1073/pnas.1321032111
MImport into Mendeley
Experimental method

Structure validation

RfreeClashscoreRamachandran outliersSidechain outliersRSRZ outliers0.24670.6%8.7%8.9%MetricValuePercentile RanksWorseBetterPercentile relative to all X-ray structuresPercentile relative to X-ray structures of similar resolution
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