4NCA
Structure of Thermus thermophilus Argonaute bound to guide DNA 19-mer and target DNA in the presence of Mg2+
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRF BEAMLINE BL17U |
Synchrotron site | SSRF |
Beamline | BL17U |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2012-11-15 |
Detector | ADSC QUANTUM 315r |
Wavelength(s) | 0.97 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 110.950, 117.561, 160.380 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 48.161 - 2.489 |
R-factor | 0.2026 |
Rwork | 0.200 |
R-free | 0.24520 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.010 |
RMSD bond angle | 0.906 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | PHASER |
Refinement software | PHENIX (1.8.2_1309) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 50.000 | 50.000 | 2.590 |
High resolution limit [Å] | 2.489 | 5.380 | 2.489 |
Rmerge | 0.127 | 0.040 | 0.920 |
Number of reflections | 73777 | ||
<I/σ(I)> | 6.5 | ||
Completeness [%] | 99.9 | 99.9 | 99.5 |
Redundancy | 6.6 | 6.9 | 6 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7 | 306 | 2.6 M sodium acetate, 0.1 M Bis-Tris propane, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 306K |