4AXU

CRYSTAL STRUCTURE OF THAUMATIN FROM AN AUTO-HARVESTED CRYSTAL, control experiment

Summary for 4AXU

• Entry DOI: 10.2210/pdb4axu/pdb
• Related: 1KWN 1LR2 1LR3 1LXZ 1LY0 1PP3 1RQW 1THI 1THU 1THV 1THW 2A7I 2BLR 2BLU 2D8O 2D8P 2VHK 2VHR 2VI1 2VI2 2VI3 2VI4 2VU6 2VU7 2WBZ
• Descriptor: THAUMATIN-1 (2 entities in total)
• Functional Keywords: plant protein, automation, high-throughput crystallization, crystal mounting, crystallization microplates
• Biological source: THAUMATOCOCCUS DANIELLII (KATEMFE)
• Cellular location: Cytoplasmic vesicle: P02883
• Total number of polymer chains: 1
• Total formula weight: 22155.98

Authors

Cipriani, F.,Rower, M.,Landret, C.,Zander, U.,Felisaz, F.,Marquez, J.A. (deposition date: 2012-06-14, release date: 2012-10-24)

Primary citation

Cipriani, F.,Rower, M.,Landret, C.,Zander, U.,Felisaz, F.,Marquez, J.A.
Crystal Direct: A New Method for Automated Crystal Harvesting Based on Laser-Induced Photoablation of Thin Films
Acta Crystallogr.,Sect.D, 68:1393-, 2012

PubMed Abstract: The use of automated systems for crystallization and X-ray data collection is now widespread. However, these two steps are separated by the need to transfer crystals from crystallization supports to X-ray data-collection supports, which is a difficult manual operation. Here, a new approach is proposed called CrystalDirect (CD) which enables full automation of the crystal-harvesting process. In this approach, crystals are grown on ultrathin films in a newly designed vapour-diffusion crystallization plate and are recovered by excision of the film through laser-induced photoablation. The film pieces containing crystals are then directly attached to a pin for X-ray data collection. This new method eliminates the delicate step of `crystal fishing', thereby enabling full automation of the crystal-mounting process. Additional advantages of this approach include the absence of mechanical stress and that it facilitates handling of microcrystals. The CD crystallization plates are also suitable for in situ crystal screening with minimal X-ray background. This method could enable the operational integration of highly automated crystallization and data-collection facilities, minimizing the delay between crystal identification and diffraction measurements. It can also contribute significantly to the advancement of challenging projects that require the systematic testing of large numbers of crystals.

PubMed: 22993093
DOI: 10.1107/S0907444912031459

Experimental method

X-RAY DIFFRACTION (1.38 Å)