4A92

Full-length HCV NS3-4A protease-helicase in complex with a macrocyclic protease inhibitor.

4A92 の概要

• エントリーDOI: 10.2210/pdb4a92/pdb
• 関連するPDBエントリー: 1A1Q 1BT7 1C2P 1CSJ 1CU1 1GX5 1GX6 1JXP 1NHU 1NHV 1NS3 1OS5 1QUV 2AWZ 2AX0 2AX1 2BRK 2BRL 2I1R 2JC0 2JC1 2WCX 2WHO 2XWY 8OHM
• 分子名称: SERINE PROTEASE NS3, ZINC ION, (1'R,2R,2'S,6S,24AS)-17-FLUORO-6-(1-METHYL-2-OXOPIPERIDINE-3-CARBOXAMIDO)-19,19-DIOXIDO-5,21,24-TRIOXO-2'-VINYL-1,2,3,5,6,7,8,9,10,11,12,13,14,20,21,23,24,24A-OCTADECAHYDROSPIRO[BENZO[S]PYRROLO[2,1-G][1,2,5,8,18]THIATETRAAZACYCLOICOSINE-22,1'-CYCLOPRO-2-CARBOXYLATEPAN]-2-YL 4-FLUOROISOINDOLINE, ... (4 entities in total)
• 機能のキーワード: hydrolase, drug design
• 由来する生物種: HEPATITIS C VIRUS (HCV)
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 143513.55

構造登録者

Schiering, N.,D'Arcy, A.,Simic, O.,Eder, J.,Raman, P.,Svergun, D.I.,Bodendorf, U. (登録日: 2011-11-23, 公開日: 2011-12-28, 最終更新日: 2024-05-08)

主引用文献

Schiering, N.,D'Arcy, A.,Villard, F.,Simic, O.,Kamke, M.,Monnet, G.,Hassiepen, U.,Svergun, D.I.,Pulfer, R.,Eder, J.,Raman, P.,Bodendorf, U.
A Macrocyclic Hcv Ns3/4A Protease Inhibitor Interacts with Protease and Helicase Residues in the Complex with its Full- Length Target.
Proc.Natl.Acad.Sci.USA, 108:21052-, 2011

PubMed Abstract: Hepatitis C virus (HCV) infection is a global health burden with over 170 million people infected worldwide. In a significant portion of patients chronic hepatitis C infection leads to serious liver diseases, including fibrosis, cirrhosis, and hepatocellular carcinoma. The HCV NS3 protein is essential for viral polyprotein processing and RNA replication and hence viral replication. It is composed of an N-terminal serine protease domain and a C-terminal helicase/NTPase domain. For full activity, the protease requires the NS4A protein as a cofactor. HCV NS3/4A protease is a prime target for developing direct-acting antiviral agents. First-generation NS3/4A protease inhibitors have recently been introduced into clinical practice, markedly changing HCV treatment options. To date, crystal structures of HCV NS3/4A protease inhibitors have only been reported in complex with the protease domain alone. Here, we present a unique structure of an inhibitor bound to the full-length, bifunctional protease-helicase NS3/4A and show that parts of the P4 capping and P2 moieties of the inhibitor interact with both protease and helicase residues. The structure sheds light on inhibitor binding to the more physiologically relevant form of the enzyme and supports exploring inhibitor-helicase interactions in the design of the next generation of HCV NS3/4A protease inhibitors. In addition, small angle X-ray scattering confirmed the observed protease-helicase domain assembly in solution.

PubMed: 22160684
DOI: 10.1073/PNAS.1110534108

実験手法

X-RAY DIFFRACTION (2.73 Å)