3Q24

X-ray crystal structure of the N4 mini-VRNAP and P2_7a promoter transcription initiation complex with pppGpG and pyrophosphate: product complex

Summary for 3Q24

• Entry DOI: 10.2210/pdb3q24/pdb
• Related: 3Q0A 3Q22 3Q23
• Descriptor: Virion RNA polymerase, DNA (5'-D(*TP*GP*CP*CP*TP*CP*CP*CP*AP*GP*GP*CP*AP*TP*CP*CP*AP*AP*AP*AP*GP*AP*AP*GP*CP*GP*GP*AP*GP*CP*TP*TP*CP*TP*TP*C)-3'), GUANOSINE-5'-TRIPHOSPHATE, ... (7 entities in total)
• Functional Keywords: protein-dna complex, two-metal catalysis, de novo transcription initiation, nucleotidyltransferase, initiation complex, dna-hairpin, virion rna polymerase, transferase-dna-rna complex, transferase/dna/rna
• Biological source: Enterobacteria phage N4 (Bacteriophage N4)
• Total number of polymer chains: 4
• Total formula weight: 269519.10

Authors

Gleghorn, M.L.,Murakami, K.S. (deposition date: 2010-12-19, release date: 2011-02-16, Last modification date: 2024-02-21)

• Primary citation: Gleghorn, M.L.,Davydova, E.K.,Basu, R.,Rothman-Denes, L.B.,Murakami, K.S.; X-ray crystal structures elucidate the nucleotidyl transfer reaction of transcript initiation using two nucleotides.; Proc.Natl.Acad.Sci.USA, 108:3566-3571, 2011; PubMed: 21321236; DOI: 10.1073/pnas.1016691108

Experimental method

X-RAY DIFFRACTION (1.81 Å)