3Q23

X-ray crystal structure of the N4 mini-VRNAP and P2_7a promoter transcription initiation complex with GMPCPP and Manganese: sustrate complex II

Summary for 3Q23

• Entry DOI: 10.2210/pdb3q23/pdb
• Related: 3Q0A 3Q22 3Q24
• Descriptor: Virion RNA polymerase, DNA (5'-D(*TP*GP*CP*CP*TP*CP*CP*CP*AP*GP*GP*CP*AP*TP*CP*CP*AP*AP*AP*AP*GP*AP*AP*GP*CP*GP*GP*AP*GP*CP*TP*TP*CP*TP*TP*C)-3'), PHOSPHOMETHYLPHOSPHONIC ACID GUANYLATE ESTER, ... (6 entities in total)
• Functional Keywords: protein-dna complex, two-metal catalysis, gmpcpp, gpcpp, de novo transcription initiation, nucleotidyltransferase, initiation complex, dna-hairpin, virion rna polymerase, phosphate ion, rna polymerase, transferase-dna complex, transferase/dna
• Biological source: Enterobacteria phage N4 (Bacteriophage N4)
• Total number of polymer chains: 4
• Total formula weight: 270899.74

Authors

Gleghorn, M.L.,Murakami, K.S. (deposition date: 2010-12-19, release date: 2011-02-16, Last modification date: 2024-02-21)

• Primary citation: Gleghorn, M.L.,Davydova, E.K.,Basu, R.,Rothman-Denes, L.B.,Murakami, K.S.; X-ray crystal structures elucidate the nucleotidyl transfer reaction of transcript initiation using two nucleotides.; Proc.Natl.Acad.Sci.USA, 108:3566-3571, 2011; PubMed: 21321236; DOI: 10.1073/pnas.1016691108

Experimental method

X-RAY DIFFRACTION (1.8 Å)