3Q23

X-ray crystal structure of the N4 mini-VRNAP and P2_7a promoter transcription initiation complex with GMPCPP and Manganese: sustrate complex II

Summary for 3Q23

Related3Q0A 3Q22 3Q24
DescriptorVirion RNA polymerase, DNA (5'-D(*TP*GP*CP*CP*TP*CP*CP*CP*AP*GP*GP*CP*AP*TP*CP*CP*AP*AP*AP*AP*GP*AP*AP*GP*CP*GP*GP*AP*GP*CP*TP*TP*CP*TP*TP*C)-3'), PHOSPHOMETHYLPHOSPHONIC ACID GUANYLATE ESTER, ... (6 entities in total)
Functional Keywordsprotein-dna complex, two-metal catalysis, gmpcpp, gpcpp, de novo transcription initiation, nucleotidyltransferase, initiation complex, dna-hairpin, virion rna polymerase, phosphate ion, rna polymerase, transferase-dna complex, transferase/dna
Biological sourceEnterobacteria phage N4 (Bacteriophage N4)
Total number of polymer chains4
Total molecular weight270899.74
Authors
Gleghorn, M.L.,Murakami, K.S. (deposition date: 2010-12-19, release date: 2011-02-16, Last modification date: 2019-07-17)
Primary citation
Gleghorn, M.L.,Davydova, E.K.,Basu, R.,Rothman-Denes, L.B.,Murakami, K.S.
X-ray crystal structures elucidate the nucleotidyl transfer reaction of transcript initiation using two nucleotides.
Proc.Natl.Acad.Sci.USA, 108:3566-3571, 2011
PubMed: 21321236 (PDB entries with the same primary citation)
DOI: 10.1073/pnas.1016691108
MImport into Mendeley
Experimental method
X-RAY DIFFRACTION (1.8 Å)
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Structure validation

RfreeClashscoreRamachandran outliersSidechain outliersRSRZ outliers 0.2305 0.1% 3.8% 5.2%MetricValuePercentile RanksWorseBetterPercentile relative to all X-ray structuresPercentile relative to X-ray structures of similar resolution