3Q24

X-ray crystal structure of the N4 mini-VRNAP and P2_7a promoter transcription initiation complex with pppGpG and pyrophosphate: product complex

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B (A, B)	Virion RNA polymerase	polymer	1117	123036.3	2	UniProt (Q859P9) Pfam (PF21867) Pfam (PF21624) Pfam (PF21894) Pfam (PF21769)	Enterobacteria phage N4 (Bacteriophage N4)
2	C, D (C, D)	DNA (5'-D(*TP*GP*CP*CP*TP*CP*CP*CP*AP*GP*GP*CP*AP*TP*CP*CP*AP*AP*AP*AP*GP*AP*AP*GP*CP*GP*GP*AP*GP*CP*TP*TP*CP*TP*TP*C)-3')	polymer	36	11007.1	2		synthetic construct
3	E (A)	GUANOSINE-5'-TRIPHOSPHATE	non-polymer		523.2	1	Chemie (GTP) PubChem (177679032) PubChem (135400627) PubChem (135487750) PubChem (136088935) PubChem (136803486) PubChem (135398633) PubChem (135544356) PubChem (135547949) PubChem (135600366) PubChem (135749822) PubChem (136005188) PubChem (136073923) PubChem (136240914) PubChem (136664747) PubChem (136772743) PubChem (136772744) PubChem (136803487) PubChem (137286749) PubChem (164715984) PubChem (171699466)
4	F (A)	GUANOSINE-5'-MONOPHOSPHATE	non-polymer		363.2	1	Chemie (5GP)
5	G, I (A, B)	DIHYDROGENPHOSPHATE ION	non-polymer		97.0	2	Chemie (2HP) PubChem (1003)
6	H, J (A, B)	PYROPHOSPHATE 2-	non-polymer		176.0	2	Chemie (POP) PubChem (4995)
7	K, L, M, N (A, B, C, D)	water	water		18.0	2399	Chemie (HOH)

Sequence modifications

A, B: 1 - 1105 (UniProt: Q859P9)

PDB	External Database	Details
Met -11	-	expression tag
Gly -10	-	expression tag
Gly -9	-	expression tag
Ser -8	-	expression tag
His -7	-	expression tag
His -6	-	expression tag
His -5	-	expression tag
His -4	-	expression tag
His -3	-	expression tag
His -2	-	expression tag
Arg -1	-	expression tag
Ser 0	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	4
	Total formula weight	268086.8
Non-Polymers*	Number of molecules	6
	Total formula weight	1432.3
All*	Total formula weight	269519.1

*Water molecules are not included.