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3Q24

X-ray crystal structure of the N4 mini-VRNAP and P2_7a promoter transcription initiation complex with pppGpG and pyrophosphate: product complex

Experimental procedure
Experimental methodSINGLE WAVELENGTH
Source typeSYNCHROTRON
Source detailsNSLS BEAMLINE X25
Synchrotron siteNSLS
BeamlineX25
Temperature [K]100
Detector technologyCCD
Collection date2006-11-10
DetectorADSC QUANTUM 315
Spacegroup nameP 21 21 21
Unit cell lengths83.459, 111.790, 277.009
Unit cell angles90.00, 90.00, 90.00
Refinement procedure
Resolution41.490 - 1.810
R-factor0.202
Rwork0.201
R-free0.23500
Structure solution methodMOLECULAR REPLACEMENT
RMSD bond length0.007
RMSD bond angle1.096
Data reduction softwareHKL-2000
Data scaling softwareHKL-2000
Phasing softwareCNS
Refinement softwareREFMAC (5.2.0019)
Data quality characteristics
 OverallOuter shell
Low resolution limit [Å]50.0001.860
High resolution limit [Å]1.8001.800
Rmerge0.0720.728
Number of reflections233493
<I/σ(I)>10.5
Completeness [%]99.798.7
Redundancy5.94.2
Crystallization Conditions
crystal IDmethodpHtemperaturedetails
1HANGING DROP82940.1 M TRIS-HCL, 0.21 M DIBASIC AMMONIUM CITRATE, 0.09 M AMMONIUM DIHYDROGEN MONOPHOSPHATE, 25% (DROP) 21.5-22% (RESERVOIR) PEG 3350, pH 8.0, HANGING DROP, temperature 294K
Crystallization Reagents
IDcrystal IDsolution IDreagent nameconcentrationdetails
111TRIS-HCL
211DIBASIC AMMONIUM CITRATE
311AMMONIUM DIHYDROGEN MONOPHOSPHATE
411PEG 3350
512TRIS-HCL
612DIBASIC AMMONIUM CITRATE
712AMMONIUM DIHYDROGEN MONOPHOSPHATE
812PEG 3350

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PDB entries from 2024-11-20

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