2WHX

A second conformation of the NS3 protease-helicase from dengue virus

Summary for 2WHX

• Entry DOI: 10.2210/pdb2whx/pdb
• Related: 2JLQ 2JLR 2JLS 2JLU 2JLV 2JLW 2JLX 2JLY 2JLZ 2WZQ
• Descriptor: SERINE PROTEASE/NTPASE/HELICASE NS3, SERINE PROTEASE SUBUNIT NS2B, ADENOSINE-5'-DIPHOSPHATE, ... (6 entities in total)
• Functional Keywords: transcription, hydrolase, atp-binding, reticulum, nucleotidyltransferase, multifunctional enzyme, transcription regulation, ribonucleoprotein, rna-directed rna polymerase, rna replication, envelope protein
• Biological source: DENGUE VIRUS 4; More
• Cellular location: Capsid protein C: Virion (Potential). Peptide pr: Secreted (By similarity). Small envelope protein M: Virion membrane; Multi-pass membrane protein (By similarity). Envelope protein E: Virion membrane; Multi- pass membrane protein (By similarity). Non-structural protein 1: Secreted. Non-structural protein 2A-alpha: Host endoplasmic reticulum membrane; Multi-pass membrane protein (Potential). Non-structural protein 2A: Host endoplasmic reticulum membrane; Multi-pass membrane protein (Potential). Serine protease subunit NS2B: Host endoplasmic reticulum membrane; Peripheral membrane protein; Cytoplasmic side (By similarity). Serine protease NS3: Host endoplasmic reticulum membrane; Peripheral membrane protein; Cytoplasmic side (By similarity). Non-structural protein 4A: Host endoplasmic reticulum membrane; Multi-pass membrane protein (By similarity). Non-structural protein 4B: Host endoplasmic reticulum membrane; Multi-pass membrane protein (By similarity). RNA-directed RNA polymerase NS5: Host endoplasmic reticulum membrane; Peripheral membrane protein; Cytoplasmic side (By similarity): Q2YHF0 Q2YHF0
• Total number of polymer chains: 2
• Total formula weight: 71602.90

Authors

Luo, D.,Lescar, J. (deposition date: 2009-05-07, release date: 2010-04-07, Last modification date: 2011-07-13)

• Primary citation: Luo, D.,Wei, N.,Doan, D.N.,Paradkar, P.N.,Chong, Y.,Davidson, A.D.,Kotaka, M.,Lescar, J.,Vasudevan, S.G.; Flexibility between the Protease and Helicase Domains of the Dengue Virus Ns3 Protein Conferred by the Linker Region and its Functional Implications.; J.Biol.Chem., 285:18817-, 2010; PubMed: 20375022; DOI: 10.1074/JBC.M109.090936

Experimental method

X-RAY DIFFRACTION (2.2 Å)