2WHX
A second conformation of the NS3 protease-helicase from dengue virus
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSRRC |
| Synchrotron site | NSRRC |
| Temperature [K] | 100 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 52.420, 87.720, 75.779 |
| Unit cell angles | 90.00, 92.90, 90.00 |
Refinement procedure
| Resolution | 19.870 - 2.200 |
| R-factor | 0.23031 |
| Rwork | 0.228 |
| R-free | 0.27233 |
| Structure solution method | OTHER |
| Starting model (for MR) | NONE |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.148 |
| Refinement software | REFMAC (5.5.0072) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 20.000 |
| High resolution limit [Å] | 2.200 |
| Rmerge | 0.080 |
| Number of reflections | 31361 |
| <I/σ(I)> | 16.5 |
| Completeness [%] | 90.4 |
| Redundancy | 4.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 7.4 | pH 7.4 |
