2JLV

Dengue virus 4 NS3 helicase in complex with ssRNA and AMPPNP

Summary for 2JLV

• Entry DOI: 10.2210/pdb2jlv/pdb
• Related: 2JLQ 2JLR 2JLS 2JLU 2JLW 2JLX 2JLY 2JLZ
• Descriptor: SERINE PROTEASE SUBUNIT NS3, 5'-R(*AP*GP*AP*CP*UP*AP*AP*CP*AP*AP*CP*U)-3', PHOSPHOAMINOPHOSPHONIC ACID-ADENYLATE ESTER, ... (7 entities in total)
• Functional Keywords: hydrolase-rna complex, ribonucleoprotein, nucleotide-binding, helicase, protease, hydrolase, atp analog, transferase, viral nucleoprotein, endoplasmic reticulum, cleavage on pair of basic residues, ssrna, virion, atpase, nucleus, membrane, secreted, multifunctional enzyme, transcription regulation, nucleotidyltransferase, ns3 helicase structure, atp-binding, rna-binding, flaviviruses, glycoprotein, rna-directed rna polymerase, rna replication, serine protease, envelope protein, dengue virus, metal-binding, transmembrane, transcription, phosphoprotein, capsid protein, hydrolase/rna
• Biological source: DENGUE VIRUS 4
• Cellular location: Capsid protein C: Virion (Potential). Peptide pr: Secreted (By similarity). Small envelope protein M: Virion membrane; Multi-pass membrane protein (By similarity). Envelope protein E: Virion membrane; Multi- pass membrane protein (By similarity). Non-structural protein 1: Secreted. Non-structural protein 2A-alpha: Host endoplasmic reticulum membrane; Multi-pass membrane protein (Potential). Non-structural protein 2A: Host endoplasmic reticulum membrane; Multi-pass membrane protein (Potential). Serine protease subunit NS2B: Host endoplasmic reticulum membrane; Peripheral membrane protein; Cytoplasmic side (By similarity). Serine protease NS3: Host endoplasmic reticulum membrane; Peripheral membrane protein; Cytoplasmic side (By similarity). Non-structural protein 4A: Host endoplasmic reticulum membrane; Multi-pass membrane protein (By similarity). Non-structural protein 4B: Host endoplasmic reticulum membrane; Multi-pass membrane protein (By similarity). RNA-directed RNA polymerase NS5: Host endoplasmic reticulum membrane; Peripheral membrane protein; Cytoplasmic side (By similarity): Q2YHF0
• Total number of polymer chains: 4
• Total formula weight: 111715.14

Authors

Luo, D.H.,Xu, T.,Watson, R.P.,Becker, D.S.,Sampath, A.,Jahnke, W.,Yeong, S.S.,Wang, C.H.,Lim, S.P.,Vasudevan, S.G.,Lescar, J. (deposition date: 2008-09-15, release date: 2008-11-25, Last modification date: 2023-12-13)

• Primary citation: Luo, D.H.,Xu, T.,Watson, R.P.,Becker, D.S.,Sampath, A.,Jahnke, W.,Yeong, S.S.,Wang, C.H.,Lim, S.P.,Strongin, A.,Vasudevan, S.G.,Lescar, J.; Insights Into RNA Unwinding and ATP Hydrolysis by the Flavivirus Ns3 Protein; Embo J., 27:3209-, 2008; PubMed: 19008861; DOI: 10.1038/EMBOJ.2008.232

Experimental method

X-RAY DIFFRACTION (1.9 Å)