2JLX

Dengue virus 4 NS3 helicase in complex with ssRNA and ADP-Vanadate

Summary for 2JLX

• Entry DOI: 10.2210/pdb2jlx/pdb
• Related: 2JLQ 2JLR 2JLS 2JLU 2JLV 2JLW 2JLY 2JLZ
• Descriptor: SERINE PROTEASE SUBUNIT NS3, 5'-R(*AP*GP*AP*CP*UP*AP*AP*CP*AP*AP*CP*U)-3', ADENOSINE-5'-DIPHOSPHATE, ... (7 entities in total)
• Functional Keywords: hydrolase-rna complex, hydrolase rna complex, envelope protein, ribonucleoprotein, capsid protein, rna replication, serine protease, glycoprotein, dengue virus, metal-binding, transferase, atp-binding, rna-binding, flaviviruses, transcription regulation, rna-directed rna polymerase, nucleotide-binding, viral nucleoprotein, secreted, helicase, vanadate, protease, hydrolase, cleavage on pair of basic residues, endoplasmic reticulum, nucleotidyltransferase, adp, ssrna, virion, atpase, nucleus, membrane, transmembrane, transcription, phosphoprotein, ns3 helicase structure, multifunctional enzyme, hydrolase/rna
• Biological source: DENGUE VIRUS 4
• Cellular location: Capsid protein C: Virion (Potential). Peptide pr: Secreted (By similarity). Small envelope protein M: Virion membrane; Multi-pass membrane protein (By similarity). Envelope protein E: Virion membrane; Multi- pass membrane protein (By similarity). Non-structural protein 1: Secreted. Non-structural protein 2A-alpha: Host endoplasmic reticulum membrane; Multi-pass membrane protein (Potential). Non-structural protein 2A: Host endoplasmic reticulum membrane; Multi-pass membrane protein (Potential). Serine protease subunit NS2B: Host endoplasmic reticulum membrane; Peripheral membrane protein; Cytoplasmic side (By similarity). Serine protease NS3: Host endoplasmic reticulum membrane; Peripheral membrane protein; Cytoplasmic side (By similarity). Non-structural protein 4A: Host endoplasmic reticulum membrane; Multi-pass membrane protein (By similarity). Non-structural protein 4B: Host endoplasmic reticulum membrane; Multi-pass membrane protein (By similarity). RNA-directed RNA polymerase NS5: Host endoplasmic reticulum membrane; Peripheral membrane protein; Cytoplasmic side (By similarity): Q2YHF0
• Total number of polymer chains: 4
• Total formula weight: 111567.38

Authors

Luo, D.H.,Xu, T.,Watson, R.P.,Becker, D.S.,Sampath, A.,Jahnke, W.,Yeong, S.S.,Wang, C.H.,Lim, S.P.,Vasudevan, S.G.,Lescar, J. (deposition date: 2008-09-15, release date: 2008-11-25, Last modification date: 2023-12-13)

• Primary citation: Luo, D.H.,Xu, T.,Watson, R.P.,Scherer-Becker, D.,Sampath, A.,Jahnke, W.,Yeong, S.S.,Wang, C.H.,Lim, S.P.,Strongin, A.,Vasudevan, S.G.,Lescar, J.; Insights Into RNA Unwinding and ATP Hydrolysis by the Flavivirus Ns3 Protein.; Embo J., 27:3209-, 2008; PubMed: 19008861; DOI: 10.1038/EMBOJ.2008.232

Experimental method

X-RAY DIFFRACTION (2.2 Å)