2JLX

Dengue virus 4 NS3 helicase in complex with ssRNA and ADP-Vanadate

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID14-4
Synchrotron site	ESRF
Beamline	ID14-4
Temperature [K]	100
Detector technology	CCD
Detector	ADSC CCD
Spacegroup name	C 1 2 1
Unit cell lengths	132.537, 105.207, 72.352
Unit cell angles	90.00, 117.62, 90.00

Refinement procedure

Resolution	20.000 - 2.200
R-factor	0.19819
Rwork	0.196
R-free	0.24506
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2jlu
RMSD bond length	0.009
RMSD bond angle	1.223
Data reduction software	MOSFLM
Data scaling software	SCALA
Refinement software	REFMAC (5.2.0019)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	78.300	2.300
High resolution limit [Å]	2.200	2.200
Rmerge	0.100	0.500
Number of reflections	127207
<I/σ(I)>	11.6	2.7
Completeness [%]	98.8	99.7
Redundancy	2.9	2.9

Crystallization Conditions

crystal ID	method	pH	temperature	details
1