2VEL
Structure-based enzyme engineering efforts with an inactive monomeric TIM variant: the importance of a single point mutation for generating an active site with suitable binding properties
Summary for 2VEL
Entry DOI | 10.2210/pdb2vel/pdb |
Related | 1AG1 1DKW 1IIG 1IIH 1KV5 1ML1 1MSS 1MTM 1TPD 1TPE 1TPF 1TRD 1TRI 1TSI 1TTI 1TTJ 2J24 2J27 2V0T 2V2C 2V2D 2V2H 2V5L 2VEI 2VEK 3TIM 4TIM 5TIM 6TIM |
Descriptor | GLYCOSOMAL TRIOSEPHOSPHATE ISOMERASE, CHLORIDE ION, 2-PHOSPHOGLYCOLIC ACID, ... (4 entities in total) |
Functional Keywords | isomerase, triosephosphate isomerase, tim barrel, glycolysis, engineering, pentose shunt, binding pocket, gluconeogenesis, lipid synthesis, substrate specificity, fatty acid biosynthesis, tim, enzyme, monomeric, glycosome |
Biological source | TRYPANOSOMA BRUCEI BRUCEI |
Total number of polymer chains | 2 |
Total formula weight | 51701.55 |
Authors | Alahuhta, M.,Salin, M.,Casteleijn, M.G.,Kemmer, C.,El-Sayed, I.,Augustyns, K.,Neubauer, P.,Wierenga, R.K. (deposition date: 2007-10-24, release date: 2008-02-19, Last modification date: 2023-12-13) |
Primary citation | Alahuhta, M.,Salin, M.,Casteleijn, M.G.,Kemmer, C.,El-Sayed, I.,Augustyns, K.,Neubauer, P.,Wierenga, R.K. Structure-Based Protein Engineering Efforts with a Monomeric Tim Variant: The Importance of a Single Point Mutation for Generating an Active Site with Suitable Binding Properties. Protein Eng.Des.Sel., 21:257-, 2008 Cited by PubMed: 18239072DOI: 10.1093/PROTEIN/GZN002 PDB entries with the same primary citation |
Experimental method | X-RAY DIFFRACTION (2.3 Å) |
Structure validation
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