2VEK

Structure-based enzyme engineering efforts with an inactive monomeric TIM variant: the importance of a single point mutation for generating an active site with suitable binding properties

2VEK の概要

• エントリーDOI: 10.2210/pdb2vek/pdb
• 関連するPDBエントリー: 1AG1 1DKW 1IIG 1IIH 1KV5 1ML1 1MSS 1MTM 1TPD 1TPE 1TPF 1TRD 1TRI 1TSI 1TTI 1TTJ 2J24 2J27 2V0T 2V2C 2V2D 2V2H 2V5L 2VEI 2VEL 2VEM 2VEN 3TIM 4TIM 5TIM 6TIM
• 分子名称: TRIOSEPHOSPHATE ISOMERASE, CITRIC ACID, TERTIARY-BUTYL ALCOHOL, ... (5 entities in total)
• 機能のキーワード: isomerase, triosephosphate isomerase, tim barrel, glycolysis, engineering, pentose shunt, binding pocket, gluconeogenesis, lipid synthesis, substrate specificity, fatty acid biosynthesis, tim, enzyme, monomeric, glycosome
• 由来する生物種: TRYPANOSOMA BRUCEI BRUCEI
• 細胞内の位置: Glycosome: P04789
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 51971.20

構造登録者

Alahuhta, M.,Salin, M.,Casteleijn, M.G.,Kemmer, C.,El-Sayed, I.,Augustyns, K.,Neubauer, P.,Wierenga, R.K. (登録日: 2007-10-24, 公開日: 2008-02-19, 最終更新日: 2023-12-13)

• 主引用文献: Alahuhta, M.,Salin, M.,Casteleijn, M.G.,Kemmer, C.,El-Sayed, I.,Augustyns, K.,Neubauer, P.,Wierenga, R.K.; Structure-Based Protein Engineering Efforts with a Monomeric Tim Variant: The Importance of a Single Point Mutation for Generating an Active Site with Suitable Binding Properties.; Protein Eng.Des.Sel., 21:257-, 2008; PubMed: 18239072; DOI: 10.1093/PROTEIN/GZN002

実験手法

X-RAY DIFFRACTION (1.6 Å)