1M78

CANDIDA ALBICANS DIHYDROFOLATE REDUCTASE COMPLEXED WITH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE (NADPH) AND 5-CHLORYL-2,4,6-QUINAZOLINETRIAMINE (GW1225)

Summary for 1M78

• Entry DOI: 10.2210/pdb1m78/pdb
• Related: 1AI9 1AOE 1IA1 1IA2 1IA3 1IA4 1M79 1M7A
• Descriptor: DIHYDROFOLATE REDUCTASE, NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE, 5-CHLORYL-2,4,6-QUINAZOLINETRIAMINE, ... (4 entities in total)
• Functional Keywords: oxidoreductase, antifungal target, reductase
• Biological source: Candida albicans
• Total number of polymer chains: 2
• Total formula weight: 46299.17

Authors

Whitlow, M.,Howard, A.J.,Kuyper, L.F. (deposition date: 2002-07-19, release date: 2003-03-04, Last modification date: 2023-08-23)

Primary citation

Whitlow, M.,Howard, A.J.,Stewart, D.,Hardman, K.D.,Kuyper, L.F.,Baccanari, D.P.,Fling, M.E.,Tansik, R.L.
X-Ray Crystallographic Studies of Candida Albicans Dihydrofolate Reductase. High Resolution Structures of the Holoenzyme and an Inhibited Ternary Complex.
J.Biol.Chem., 272:30289-30298, 1997

PubMed Abstract: The recent rise in systemic fungal infections has created a need for the development of new antifungal agents. As part of an effort to provide therapeutically effective inhibitors of fungal dihydrofolate reductase (DHFR), we have cloned, expressed, purified, crystallized, and determined the three-dimensional structure of Candida albicans DHFR. The 192-residue enzyme, which was expressed in Escherichia coli and purified by methotrexate affinity and cation exchange chromatography, was 27% identical to human DHFR. Crystals of C. albicans DHFR were grown as the holoenzyme complex and as a ternary complex containing a pyrroloquinazoline inhibitor. Both complexes crystallized with two molecules in the asymmetric unit in space group P21. The final structures had R-factors of 0.199 at 1.85-A resolution and 0.155 at 1.60-A resolution, respectively. The enzyme fold was similar to that of bacterial and vertebrate DHFR, and the binding of a nonselective diaminopyrroloquinazoline inhibitor and the interactions of NADPH with protein were typical of ligand binding to other DHFRs. However, the width of the active site cleft of C. albicans DHFR was significantly larger than that of the human enzyme, providing a basis for the design of potentially selective inhibitors.

PubMed: 9374515
DOI: 10.1074/jbc.272.48.30289

Experimental method

X-RAY DIFFRACTION (1.71 Å)