A Second Divalent Metal Ion in the Active Site of a New Crystal Form of Human Apurinic/Apyridinimic Endonuclease, Ape1, and its Implications for the Catalytic Mechanism

Summary for 1HD7

Related1BIX 1CQG 1CQH 1DE8 1DE9 1DEW 1E9N
DescriptorDNA-(APURINIC OR APYRIMIDINIC SITE) LYASE, LEAD (II) ION (3 entities in total)
Functional Keywordsdna repair, endonuclease, ape1, hap1, ref-1
Biological sourceHOMO SAPIENS (HUMAN)
Cellular locationNucleus. DNA-(apurinic or apyrimidinic site) lyase, mitochondrial: Mitochondrion P27695
Total number of polymer chains1
Total molecular weight35813.69
Beernink, P.T.,Segelke, B.W.,Rupp, B. (deposition date: 2000-11-09, release date: 2001-02-16, Last modification date: 2011-07-13)
Primary citation
Beernink, P.T.,Segelke, B.W.,Hadi, M.Z.,Erzberger, J.P.,Wilson III, D.M.,Rupp, B.
Two Divalent Metal Ions in the Active Site of a New Crystal Form of Human Apurinic/Apyrimidinic Endonuclease, Ape1: Implications for the Catalytic Mechanism
J.Mol.Biol., 307:1023-, 2001
PubMed: 11286553 (PDB entries with the same primary citation)
DOI: 10.1006/JMBI.2001.4529
MImport into Mendeley
Experimental method

Structure validation

ClashscoreRamachandran outliersSidechain outliersRSRZ outliers50.4%4.1%8.1%MetricValuePercentile RanksWorseBetterPercentile relative to all X-ray structuresPercentile relative to X-ray structures of similar resolution
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