1HD7
A Second Divalent Metal Ion in the Active Site of a New Crystal Form of Human Apurinic/Apyridinimic Endonuclease, Ape1, and its Implications for the Catalytic Mechanism
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRL BEAMLINE BL1-5 |
Synchrotron site | SSRL |
Beamline | BL1-5 |
Temperature [K] | 110 |
Detector technology | CCD |
Collection date | 1999-03-15 |
Detector | ADSC CCD |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 128.390, 44.850, 78.140 |
Unit cell angles | 90.00, 124.54, 90.00 |
Refinement procedure
Resolution | 25.000 - 1.950 |
R-factor | 0.205 * |
Rwork | 0.205 |
R-free | 0.25500 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | TO BE PUBLISHED |
RMSD bond length | 0.016 |
RMSD bond angle | 0.038 |
Data reduction software | MOSFLM |
Data scaling software | SCALA |
Phasing software | EPMR |
Refinement software | REFMAC |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 25.000 | 2.010 |
High resolution limit [Å] | 1.950 | 1.950 |
Rmerge | 0.074 * | 0.471 * |
Total number of observations | 91465 * | |
Number of reflections | 34905 | |
<I/σ(I)> | 6.6 | 1.5 |
Completeness [%] | 99.4 | 99.3 * |
Redundancy | 3.3 | 3.2 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | Vapor diffusion, hanging drop * | 4.6 * | 4 * | 0.1M NAOAC PH 4.6, 25% PEG 4K, 1 MM PB(II)OAC, 10-12 MG/ML PROTEIN. |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | drop | protein | 10-12 (mg/ml) | |
2 | 1 | reservoir | sodium acetate | 0.1 (M) | |
3 | 1 | reservoir | PEG4000 | 25 (%(w/v)) | |
4 | 1 | reservoir | lead (II) acetate | 1 (mM) |