1GZS

CRYSTAL STRUCTURE OF THE COMPLEX BETWEEN THE GEF DOMAIN OF THE SALMONELLA TYPHIMURIUM SOPE TOXIN AND HUMAN Cdc42

Summary for 1GZS

• Entry DOI: 10.2210/pdb1gzs/pdb
• Related: 1A4R 1AJE 1AN0 1CEE 1CF4 1DOA 1E0A 1EES 1GRN 1KI1 1KZ7 1KZG 2NGR
• Descriptor: GTP-BINDING PROTEIN, SOPE, SULFATE ION, ... (4 entities in total)
• Functional Keywords: toxin/cell cycle, complex (toxin-cell cycle protein), sope, cdc42, salmonella typhimurium, gef, toxin, gtp- binding, lipoprotein, prenylation, toxin-cell cycle complex
• Biological source: HOMO SAPIENS; More
• Cellular location: Secreted: 1GZS
• Total number of polymer chains: 4
• Total formula weight: 76533.26

Authors

Buchwald, G.,Friebel, A.,Galan, J.E.,Hardt, W.D.,Wittinghofer, A.,Scheffzek, K. (deposition date: 2002-06-05, release date: 2002-09-12, Last modification date: 2024-05-08)

Primary citation

Buchwald, G.,Friebel, A.,Galan, J.E.,Hardt, W.D.,Wittinghofer, A.,Scheffzek, K.
Structural Basis for the Reversible Activation of a Rho Protein by the Bacterial Toxin Sope
Embo J., 21:3286-, 2002

PubMed Abstract: The bacterial enteropathogen Salmonella typhimurium employs a type III secretion system to inject bacterial toxins into the host cell cytosol. These toxins transiently activate Rho family GTP-binding protein-dependent signaling cascades to induce cytoskeletal rearrangements. One of these translocated Salmonella toxins, SopE, can activate Cdc42 in a Dbl-like fashion despite its lack of sequence similarity to Dbl-like proteins, the Rho-specific eukaryotic guanine nucleotide exchange factors. To elucidate the mechanism of SopE-mediated guanine nucleotide exchange, we have analyzed the structure of the complex between a catalytic fragment of SopE and Cdc42. SopE binds to and locks the switch I and switch II regions of Cdc42 in a conformation that promotes guanine nucleotide release. This conformation is strikingly similar to that of Rac1 in complex with the eukaryotic Dbl-like exchange factor Tiam1. However, the catalytic domain of SopE has an entirely different architecture from that of Tiam1 and interacts with the switch regions via different amino acids. Therefore, SopE represents the first example of a non-Dbl-like protein capable of inducing guanine nucleotide exchange in Rho family proteins.

PubMed: 12093730
DOI: 10.1093/EMBOJ/CDF329

Experimental method

X-RAY DIFFRACTION (2.3 Å)