1KI1
Guanine Nucleotide Exchange Region of Intersectin in Complex with Cdc42
Summary for 1KI1
| Entry DOI | 10.2210/pdb1ki1/pdb |
| Descriptor | G25K GTP-binding protein, placental isoform, intersectin long form, SULFATE ION, ... (4 entities in total) |
| Functional Keywords | protein-protein complex, dh domain, ph domain, rho gtpase, signaling protein |
| Biological source | Homo sapiens (human) More |
| Cellular location | Cell membrane; Lipid-anchor; Cytoplasmic side (Potential): P60953 Endomembrane system (By similarity): Q15811 |
| Total number of polymer chains | 4 |
| Total formula weight | 123949.31 |
| Authors | Snyder, J.T.,Pruitt, W.M.,Der, C.J.,Sondek, J. (deposition date: 2001-12-02, release date: 2002-05-29, Last modification date: 2024-02-14) |
| Primary citation | Snyder, J.T.,Worthylake, D.K.,Rossman, K.L.,Betts, L.,Pruitt, W.M.,Siderovski, D.P.,Der, C.J.,Sondek, J. Structural basis for the selective activation of Rho GTPases by Dbl exchange factors. Nat.Struct.Biol., 9:468-475, 2002 Cited by PubMed Abstract: Activation of Rho-family GTPases involves the removal of bound GDP and the subsequent loading of GTP, all catalyzed by guanine nucleotide exchange factors (GEFs) of the Dbl-family. Despite high sequence conservation among Rho GTPases, Dbl proteins possess a wide spectrum of discriminatory potentials for Rho-family members. To rationalize this specificity, we have determined crystal structures of the conserved, catalytic fragments (Dbl and pleckstrin homology domains) of the exchange factors intersectin and Dbs in complex with their cognate GTPases, Cdc42 and RhoA, respectively. Structure-based mutagenesis of intersectin and Dbs reveals the key determinants responsible for promoting exchange activity in Cdc42, Rac1 and RhoA. These findings provide critical insight into the structural features necessary for the proper pairing of Dbl-exchange factors with Rho GTPases and now allow for the detailed manipulation of signaling pathways mediated by these oncoproteins in vivo. PubMed: 12006984DOI: 10.1038/nsb796 PDB entries with the same primary citation |
| Experimental method | X-RAY DIFFRACTION (2.3 Å) |
Structure validation
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