1KI1

Guanine Nucleotide Exchange Region of Intersectin in Complex with Cdc42

Experimental procedure

Experimental method	MAD
Source type	SYNCHROTRON
Source details	NSLS BEAMLINE X4A
Synchrotron site	NSLS
Beamline	X4A
Temperature [K]	100
Detector technology	CCD
Collection date	2000-08-15
Detector	ADSC QUANTUM 4
Wavelength(s)	0.9787, 0.9792
Spacegroup name	P 1 21 1
Unit cell lengths	85.370, 79.230, 116.410
Unit cell angles	90.00, 111.51, 90.00

Refinement procedure

Resolution	20.000 * - 2.300
R-factor	0.231 *
Rwork	0.231
R-free	0.24700
Structure solution method	MAD
RMSD bond length	0.009
RMSD bond angle	1.416 *
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	SHELXS
Refinement software	CNS

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	30.000	2.380
High resolution limit [Å]	2.300	2.300
Rmerge	0.076 *	0.236 *
Total number of observations	572588 *
Number of reflections	55366 *
<I/σ(I)>	34	4.2
Completeness [%]	85.3 *	79.6 *

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, hanging drop *	7.5	4 *	PEG 4000, ammonium sulfate, Tris, pH 7.5, VAPOR DIFFUSION, SITTING DROP, temperature 277K

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	20 (mg/ml)
2	1	reservoir	PEG4000	15 (%(w/w))
3	1	reservoir	Tris	100 (mM)	pH7.5
4	1	reservoir	ammonium sulfate	150 (mM)
5	1	reservoir	dithiothreitol	1 (mM)