1HF4

STRUCTURAL EFFECTS OF MONOVALENT ANIONS ON POLYMORPHIC LYSOZYME CRYSTALS

Summary for 1HF4

• Entry DOI: 10.2210/pdb1hf4/pdb
• Related: 193L 194L 1A2Y 1AKI 1AT5 1AT6 1AZF 1B0D 1B2K 1BGI 1BHZ 1BVK 1BVX 1BWH 1BWI 1BWJ 1C08 1C10 1DPW 1DPX 1DQJ 1E8L 1F0W 1F10 1F3J 1FLQ 1FLU 1FLW 1FLY 1FN5 1HSW 1HSX 1JPO 1KIP 1KIQ 1KIR 1KXW 1KXX 1KXY 1LCN 1LKR 1LKS 1LPI 1LYO 1LZ8 1LZN 1MEL 1QTK 1RFP 1UCO 1UIA 1UIB 1UIC 1UID 1UIE 1UIF 1UIG 1UIH 1XEI 1XEJ 1XEK 2LYO 3LYO 3LZT 4LYO 4LZT
• Descriptor: LYSOZYME, SODIUM ION, NITRATE ION, ... (4 entities in total)
• Functional Keywords: hydrolase, hydrolase (o-glycosyl)
• Biological source: GALLUS GALLUS (CHICKEN)
• Total number of polymer chains: 2
• Total formula weight: 29204.34

Authors

Vaney, M.C.,Broutin, I.,Ries-Kautt, M.,Ducruix, A. (deposition date: 2000-11-29, release date: 2001-01-07, Last modification date: 2024-11-06)

Primary citation

Vaney, M.C.,Broutin, I.,Retailleau, P.,Douangamath, A.,Lafont, S.,Hamiaux, C.,Prange, T.,Ducruix, A.,Ries-Kautt, M.
Structural Effects of Monovalent Anions on Polymorphic Lysozyme Crystals
Acta Crystallogr.,Sect.D, 57:929-, 2001

PubMed Abstract: Understanding direct salt effects on protein crystal polymorphism is addressed by comparing different crystal forms (triclinic, monoclinic, tetragonal and orthorhombic) for hen, turkey, bob white quail and human lysozymes. Four new structures of hen egg-white lysozyme are reported: crystals grown in the presence of NapTS diffracted to 1.85 A, of NaI to 1.6 A, of NaNO(3) to 1.45 A and of KSCN to 1.63 A. These new structures are compared with previously published structures in order to draw a mapping of the surface of different lysozymes interacting with monovalent anions, such as nitrate, chloride, iodide, bromide and thiocyanate. An analysis of the structural sites of these anions in the various lysozyme structures is presented. This study shows common anion sites whatever the crystal form and the chemical nature of anions, while others seem specific to a given geometry and a particular charge environment induced by the crystal packing.

PubMed: 11418760
DOI: 10.1107/S0907444901004504

Experimental method

X-RAY DIFFRACTION (1.45 Å)