1LZ8

LYSOZYME PHASED ON ANOMALOUS SIGNAL OF SULFURS AND CHLORINES

Summary for 1LZ8

Entry DOI	10.2210/pdb1lz8/pdb
Descriptor	PROTEIN (LYSOZYME), CHLORIDE ION, SODIUM ION, ... (4 entities in total)
Functional Keywords	hydrolase, o-glycosyl, glycosidase
Biological source	Gallus gallus (chicken)
Cellular location	Secreted: P00698
Total number of polymer chains	1
Total formula weight	14637.77
Authors	Dauter, Z.,Dauter, M.,De La Fortelle, E.,Bricogne, G.,Sheldrick, G.M. (deposition date: 1999-03-14, release date: 1999-05-26, Last modification date: 2024-11-20)
Primary citation	Dauter, Z.,Dauter, M.,de La Fortelle, E.,Bricogne, G.,Sheldrick, G.M. Can anomalous signal of sulfur become a tool for solving protein crystal structures? J.Mol.Biol., 289:83-92, 1999 Cited by PubMed Abstract: A general method for solving the phase problem from native crystals of macromolecules has long eluded structural biology. For well diffracting crystals this goal can now be achieved, as is shown here, thanks to modern data collection techniques and new statistical phasing algorithms. Using solely a native crystal of tetragonal hen egg-white lysozyme, a protein of 14 kDa molecular mass, it was possible to detect the positions of the ten sulfur and seven chlorine atoms from their anomalous signal, and proceed from there to obtain an electron-density map of very high quality. PubMed: 10339407 DOI: 10.1006/jmbi.1999.2743 PDB entries with the same primary citation
Experimental method	X-RAY DIFFRACTION (1.53 Å)

Structure validation