7USE
Cryo-EM structure of WAVE regulatory complex with Rac1 bound on both A and D site
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A (A) | Cytoplasmic FMR1-interacting protein 1 | polymer | 1253 | 145363.8 | 1 | UniProt (Q7L576) Pfam (PF07159) Pfam (PF05994) | Homo sapiens (human) | Specifically Rac1-associated protein 1,Sra-1,p140sra-1 |
| 2 | B (B) | Nck-associated protein 1 | polymer | 1128 | 128940.7 | 1 | UniProt (Q9Y2A7) Pfam (PF09735) | Homo sapiens (human) | NAP 1,Membrane-associated protein HEM-2,p125Nap1 |
| 3 | C (C) | Wiskott-Aldrich syndrome protein family member 1 | polymer | 323 | 37009.4 | 1 | UniProt (Q92558) Pfam (PF02205) | Homo sapiens (human) | WASP family protein member 1,Protein WAVE-1,Verprolin homology domain-containing protein 1 |
| 4 | D (D) | Protein BRICK1 | polymer | 75 | 8756.9 | 1 | UniProt (Q8WUW1) | Homo sapiens (human) | BRK1 |
| 5 | E (E) | Abl interactor 2 | polymer | 158 | 18041.5 | 1 | UniProt (E9PEZ7) Pfam (PF07815) UniProt (by SIFTS) (Q9NYB9) | Homo sapiens (human) | |
| 6 | F (F) | Ras-related C3 botulinum toxin substrate 1 | polymer | 188 | 21025.5 | 1 | UniProt (P63000) Pfam (PF00071) | Homo sapiens (human) | Cell migration-inducing gene 5 protein,Ras-like protein TC25,p21-Rac1 |
| 7 | G (G) | Ras-related C3 botulinum toxin substrate 1 | polymer | 188 | 21010.5 | 1 | UniProt (P63000) Pfam (PF00071) | Homo sapiens (human) | Cell migration-inducing gene 5 protein,Ras-like protein TC25,p21-Rac1 |
| 8 | H (F) | PHOSPHOAMINOPHOSPHONIC ACID-GUANYLATE ESTER | non-polymer | 522.2 | 1 | Chemie (GNP) | |||
| 9 | I, K (F, G) | MAGNESIUM ION | non-polymer | 24.3 | 2 | Chemie (MG) | |||
| 10 | J (G) | GUANOSINE-5'-TRIPHOSPHATE | non-polymer | 523.2 | 1 | Chemie (GTP) |
Sequence modifications
C: 1 - 230 (UniProt: Q92558)
C: 249 - 323 (UniProt: Q92558)
F: 1 - 188 (UniProt: P63000)
G: 1 - 188 (UniProt: P63000)
C: 249 - 323 (UniProt: Q92558)
| PDB | External Database | Details |
|---|---|---|
| Gly 231 | - | linker |
| Gly 232 | - | linker |
| Ser 233 | - | linker |
| Gly 234 | - | linker |
| Gly 235 | - | linker |
| Ser 236 | - | linker |
| Gly 237 | - | linker |
| Gly 238 | - | linker |
| Ser 239 | - | linker |
| Gly 240 | - | linker |
| Gly 241 | - | linker |
| Ser 242 | - | linker |
| Gly 243 | - | linker |
| Gly 244 | - | linker |
| Ser 245 | - | linker |
| Gly 246 | - | linker |
| Gly 247 | - | linker |
| Ser 248 | - | linker |
| PDB | External Database | Details |
|---|---|---|
| Ser 29 | Pro 29 | engineered mutation |
| PDB | External Database | Details |
|---|---|---|
| Ser 29 | Pro 29 | engineered mutation |
| Leu 61 | Gln 61 | engineered mutation |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 7 |
| Total formula weight | 380148.2 | |
| Non-Polymers* | Number of molecules | 4 |
| Total formula weight | 1094.0 | |
| All* | Total formula weight | 381242.2 |
