7USE

Cryo-EM structure of WAVE regulatory complex with Rac1 bound on both A and D site

Functional Information from GO Data

Chain	GOid	namespace	contents
A	0000340	molecular_function	RNA 7-methylguanosine cap binding
A	0000902	biological_process	cell morphogenesis
A	0001726	cellular_component	ruffle
A	0003779	molecular_function	actin binding
A	0005515	molecular_function	protein binding
A	0005576	cellular_component	extracellular region
A	0005737	cellular_component	cytoplasm
A	0005829	cellular_component	cytosol
A	0005925	cellular_component	focal adhesion
A	0006417	biological_process	regulation of translation
A	0007399	biological_process	nervous system development
A	0007411	biological_process	axon guidance
A	0008360	biological_process	regulation of cell shape
A	0010592	biological_process	positive regulation of lamellipodium assembly
A	0016601	biological_process	Rac protein signal transduction
A	0017148	biological_process	negative regulation of translation
A	0030027	cellular_component	lamellipodium
A	0030032	biological_process	lamellipodium assembly
A	0030154	biological_process	cell differentiation
A	0030371	molecular_function	translation repressor activity
A	0030833	biological_process	regulation of actin filament polymerization
A	0031175	biological_process	neuron projection development
A	0031209	cellular_component	SCAR complex
A	0031252	cellular_component	cell leading edge
A	0031267	molecular_function	small GTPase binding
A	0031529	biological_process	ruffle organization
A	0034774	cellular_component	secretory granule lumen
A	0035580	cellular_component	specific granule lumen
A	0043005	cellular_component	neuron projection
A	0045202	cellular_component	synapse
A	0048471	cellular_component	perinuclear region of cytoplasm
A	0048675	biological_process	axon extension
A	0050890	biological_process	cognition
A	0051015	molecular_function	actin filament binding
A	0051388	biological_process	positive regulation of neurotrophin TRK receptor signaling pathway
A	0060076	cellular_component	excitatory synapse
A	0070062	cellular_component	extracellular exosome
A	0097484	biological_process	dendrite extension
A	0098794	cellular_component	postsynapse
A	0099578	biological_process	regulation of translation at postsynapse, modulating synaptic transmission
A	1903502	cellular_component	translation repressor complex
A	1904724	cellular_component	tertiary granule lumen
A	1905274	biological_process	regulation of modification of postsynaptic actin cytoskeleton
A	2000601	biological_process	positive regulation of Arp2/3 complex-mediated actin nucleation
B	0000902	biological_process	cell morphogenesis
B	0001726	cellular_component	ruffle
B	0005515	molecular_function	protein binding
B	0005829	cellular_component	cytosol
B	0005886	cellular_component	plasma membrane
B	0005925	cellular_component	focal adhesion
B	0006915	biological_process	apoptotic process
B	0007417	biological_process	central nervous system development
B	0010592	biological_process	positive regulation of lamellipodium assembly
B	0016477	biological_process	cell migration
B	0016601	biological_process	Rac protein signal transduction
B	0030027	cellular_component	lamellipodium
B	0030031	biological_process	cell projection assembly
B	0030838	biological_process	positive regulation of actin filament polymerization
B	0030866	biological_process	cortical actin cytoskeleton organization
B	0031209	cellular_component	SCAR complex
B	0031258	cellular_component	lamellipodium membrane
B	0031267	molecular_function	small GTPase binding
B	0031941	cellular_component	filamentous actin
B	0048812	biological_process	neuron projection morphogenesis
B	0070062	cellular_component	extracellular exosome
B	0098794	cellular_component	postsynapse
B	2000601	biological_process	positive regulation of Arp2/3 complex-mediated actin nucleation
C	0003779	molecular_function	actin binding
C	0005856	cellular_component	cytoskeleton
C	0030036	biological_process	actin cytoskeleton organization
D	0005515	molecular_function	protein binding
D	0005829	cellular_component	cytosol
D	0005856	cellular_component	cytoskeleton
D	0007015	biological_process	actin filament organization
D	0008064	biological_process	regulation of actin polymerization or depolymerization
D	0010592	biological_process	positive regulation of lamellipodium assembly
D	0016601	biological_process	Rac protein signal transduction
D	0030027	cellular_component	lamellipodium
D	0031209	cellular_component	SCAR complex
D	0031267	molecular_function	small GTPase binding
D	0031334	biological_process	positive regulation of protein-containing complex assembly
D	0042802	molecular_function	identical protein binding
D	0044877	molecular_function	protein-containing complex binding
D	0048870	biological_process	cell motility
D	0070062	cellular_component	extracellular exosome
D	2000601	biological_process	positive regulation of Arp2/3 complex-mediated actin nucleation
F	0000166	molecular_function	nucleotide binding
F	0000242	cellular_component	pericentriolar material
F	0001764	biological_process	neuron migration
F	0001891	cellular_component	phagocytic cup
F	0001934	biological_process	positive regulation of protein phosphorylation
F	0003376	biological_process	sphingosine-1-phosphate receptor signaling pathway
F	0003924	molecular_function	GTPase activity
F	0003925	molecular_function	G protein activity
F	0005515	molecular_function	protein binding
F	0005525	molecular_function	GTP binding
F	0005634	cellular_component	nucleus
F	0005654	cellular_component	nucleoplasm
F	0005730	cellular_component	nucleolus
F	0005737	cellular_component	cytoplasm
F	0005789	cellular_component	endoplasmic reticulum membrane
F	0005802	cellular_component	trans-Golgi network
F	0005829	cellular_component	cytosol
F	0005856	cellular_component	cytoskeleton
F	0005884	cellular_component	actin filament
F	0005886	cellular_component	plasma membrane
F	0005925	cellular_component	focal adhesion
F	0005938	cellular_component	cell cortex
F	0006911	biological_process	phagocytosis, engulfment
F	0006954	biological_process	inflammatory response
F	0007015	biological_process	actin filament organization
F	0007155	biological_process	cell adhesion
F	0007160	biological_process	cell-matrix adhesion
F	0007163	biological_process	establishment or maintenance of cell polarity
F	0007167	biological_process	enzyme-linked receptor protein signaling pathway
F	0007264	biological_process	small GTPase-mediated signal transduction
F	0008045	biological_process	motor neuron axon guidance
F	0008360	biological_process	regulation of cell shape
F	0009611	biological_process	response to wounding
F	0009653	biological_process	anatomical structure morphogenesis
F	0010310	biological_process	regulation of hydrogen peroxide metabolic process
F	0010591	biological_process	regulation of lamellipodium assembly
F	0010592	biological_process	positive regulation of lamellipodium assembly
F	0010595	biological_process	positive regulation of endothelial cell migration
F	0010762	biological_process	regulation of fibroblast migration
F	0010764	biological_process	negative regulation of fibroblast migration
F	0010810	biological_process	regulation of cell-substrate adhesion
F	0016020	cellular_component	membrane
F	0016477	biological_process	cell migration
F	0016601	biological_process	Rac protein signal transduction
F	0016787	molecular_function	hydrolase activity
F	0019899	molecular_function	enzyme binding
F	0019901	molecular_function	protein kinase binding
F	0030027	cellular_component	lamellipodium
F	0030031	biological_process	cell projection assembly
F	0030032	biological_process	lamellipodium assembly
F	0030036	biological_process	actin cytoskeleton organization
F	0030041	biological_process	actin filament polymerization
F	0030334	biological_process	regulation of cell migration
F	0030425	cellular_component	dendrite
F	0030667	cellular_component	secretory granule membrane
F	0030672	cellular_component	synaptic vesicle membrane
F	0030742	molecular_function	GTP-dependent protein binding
F	0030865	biological_process	cortical cytoskeleton organization
F	0030900	biological_process	forebrain development
F	0031410	cellular_component	cytoplasmic vesicle
F	0031529	biological_process	ruffle organization
F	0031901	cellular_component	early endosome membrane
F	0031996	molecular_function	thioesterase binding
F	0032496	biological_process	response to lipopolysaccharide
F	0032587	cellular_component	ruffle membrane
F	0032707	biological_process	negative regulation of interleukin-23 production
F	0032956	biological_process	regulation of actin cytoskeleton organization
F	0034446	biological_process	substrate adhesion-dependent cell spreading
F	0035025	biological_process	positive regulation of Rho protein signal transduction
F	0035556	biological_process	intracellular signal transduction
F	0035567	biological_process	non-canonical Wnt signaling pathway
F	0035774	biological_process	positive regulation of insulin secretion involved in cellular response to glucose stimulus
F	0036464	cellular_component	cytoplasmic ribonucleoprotein granule
F	0042470	cellular_component	melanosome
F	0042554	biological_process	superoxide anion generation
F	0042734	cellular_component	presynaptic membrane
F	0042995	cellular_component	cell projection
F	0043020	cellular_component	NADPH oxidase complex
F	0043652	biological_process	engulfment of apoptotic cell
F	0044877	molecular_function	protein-containing complex binding
F	0045202	cellular_component	synapse
F	0045211	cellular_component	postsynaptic membrane
F	0045428	biological_process	regulation of nitric oxide biosynthetic process
F	0045730	biological_process	respiratory burst
F	0046425	biological_process	regulation of receptor signaling pathway via JAK-STAT
F	0048012	biological_process	hepatocyte growth factor receptor signaling pathway
F	0048261	biological_process	negative regulation of receptor-mediated endocytosis
F	0048812	biological_process	neuron projection morphogenesis
F	0048870	biological_process	cell motility
F	0051022	molecular_function	Rho GDP-dissociation inhibitor binding
F	0051492	biological_process	regulation of stress fiber assembly
F	0051496	biological_process	positive regulation of stress fiber assembly
F	0051668	biological_process	localization within membrane
F	0051894	biological_process	positive regulation of focal adhesion assembly
F	0055038	cellular_component	recycling endosome membrane
F	0060071	biological_process	Wnt signaling pathway, planar cell polarity pathway
F	0060091	cellular_component	kinocilium
F	0060263	biological_process	regulation of respiratory burst
F	0060326	biological_process	cell chemotaxis
F	0061344	biological_process	regulation of cell adhesion involved in heart morphogenesis
F	0070062	cellular_component	extracellular exosome
F	0071526	biological_process	semaphorin-plexin signaling pathway
F	0090023	biological_process	positive regulation of neutrophil chemotaxis
F	0097178	biological_process	ruffle assembly
F	0098794	cellular_component	postsynapse
F	0098978	cellular_component	glutamatergic synapse
F	0101003	cellular_component	ficolin-1-rich granule membrane
F	0150052	biological_process	regulation of postsynapse assembly
F	1900026	biological_process	positive regulation of substrate adhesion-dependent cell spreading
F	1900029	biological_process	positive regulation of ruffle assembly
F	1900242	biological_process	regulation of synaptic vesicle endocytosis
F	1902622	biological_process	regulation of neutrophil migration
F	1903348	biological_process	positive regulation of bicellular tight junction assembly
F	1904948	biological_process	midbrain dopaminergic neuron differentiation
F	2000386	biological_process	positive regulation of ovarian follicle development
G	0000166	molecular_function	nucleotide binding
G	0000242	cellular_component	pericentriolar material
G	0001764	biological_process	neuron migration
G	0001891	cellular_component	phagocytic cup
G	0001934	biological_process	positive regulation of protein phosphorylation
G	0003376	biological_process	sphingosine-1-phosphate receptor signaling pathway
G	0003924	molecular_function	GTPase activity
G	0003925	molecular_function	G protein activity
G	0005515	molecular_function	protein binding
G	0005525	molecular_function	GTP binding
G	0005634	cellular_component	nucleus
G	0005654	cellular_component	nucleoplasm
G	0005730	cellular_component	nucleolus
G	0005737	cellular_component	cytoplasm
G	0005789	cellular_component	endoplasmic reticulum membrane
G	0005802	cellular_component	trans-Golgi network
G	0005829	cellular_component	cytosol
G	0005856	cellular_component	cytoskeleton
G	0005884	cellular_component	actin filament
G	0005886	cellular_component	plasma membrane
G	0005925	cellular_component	focal adhesion
G	0005938	cellular_component	cell cortex
G	0006911	biological_process	phagocytosis, engulfment
G	0006954	biological_process	inflammatory response
G	0007015	biological_process	actin filament organization
G	0007155	biological_process	cell adhesion
G	0007160	biological_process	cell-matrix adhesion
G	0007163	biological_process	establishment or maintenance of cell polarity
G	0007167	biological_process	enzyme-linked receptor protein signaling pathway
G	0007264	biological_process	small GTPase-mediated signal transduction
G	0008045	biological_process	motor neuron axon guidance
G	0008360	biological_process	regulation of cell shape
G	0009611	biological_process	response to wounding
G	0009653	biological_process	anatomical structure morphogenesis
G	0010310	biological_process	regulation of hydrogen peroxide metabolic process
G	0010591	biological_process	regulation of lamellipodium assembly
G	0010592	biological_process	positive regulation of lamellipodium assembly
G	0010595	biological_process	positive regulation of endothelial cell migration
G	0010762	biological_process	regulation of fibroblast migration
G	0010764	biological_process	negative regulation of fibroblast migration
G	0010810	biological_process	regulation of cell-substrate adhesion
G	0016020	cellular_component	membrane
G	0016477	biological_process	cell migration
G	0016601	biological_process	Rac protein signal transduction
G	0016787	molecular_function	hydrolase activity
G	0019899	molecular_function	enzyme binding
G	0019901	molecular_function	protein kinase binding
G	0030027	cellular_component	lamellipodium
G	0030031	biological_process	cell projection assembly
G	0030032	biological_process	lamellipodium assembly
G	0030036	biological_process	actin cytoskeleton organization
G	0030041	biological_process	actin filament polymerization
G	0030334	biological_process	regulation of cell migration
G	0030425	cellular_component	dendrite
G	0030667	cellular_component	secretory granule membrane
G	0030672	cellular_component	synaptic vesicle membrane
G	0030742	molecular_function	GTP-dependent protein binding
G	0030865	biological_process	cortical cytoskeleton organization
G	0030900	biological_process	forebrain development
G	0031410	cellular_component	cytoplasmic vesicle
G	0031529	biological_process	ruffle organization
G	0031901	cellular_component	early endosome membrane
G	0031996	molecular_function	thioesterase binding
G	0032496	biological_process	response to lipopolysaccharide
G	0032587	cellular_component	ruffle membrane
G	0032707	biological_process	negative regulation of interleukin-23 production
G	0032956	biological_process	regulation of actin cytoskeleton organization
G	0034446	biological_process	substrate adhesion-dependent cell spreading
G	0035025	biological_process	positive regulation of Rho protein signal transduction
G	0035556	biological_process	intracellular signal transduction
G	0035567	biological_process	non-canonical Wnt signaling pathway
G	0035774	biological_process	positive regulation of insulin secretion involved in cellular response to glucose stimulus
G	0036464	cellular_component	cytoplasmic ribonucleoprotein granule
G	0042470	cellular_component	melanosome
G	0042554	biological_process	superoxide anion generation
G	0042734	cellular_component	presynaptic membrane
G	0042995	cellular_component	cell projection
G	0043020	cellular_component	NADPH oxidase complex
G	0043652	biological_process	engulfment of apoptotic cell
G	0044877	molecular_function	protein-containing complex binding
G	0045202	cellular_component	synapse
G	0045211	cellular_component	postsynaptic membrane
G	0045428	biological_process	regulation of nitric oxide biosynthetic process
G	0045730	biological_process	respiratory burst
G	0046425	biological_process	regulation of receptor signaling pathway via JAK-STAT
G	0048012	biological_process	hepatocyte growth factor receptor signaling pathway
G	0048261	biological_process	negative regulation of receptor-mediated endocytosis
G	0048812	biological_process	neuron projection morphogenesis
G	0048870	biological_process	cell motility
G	0051022	molecular_function	Rho GDP-dissociation inhibitor binding
G	0051492	biological_process	regulation of stress fiber assembly
G	0051496	biological_process	positive regulation of stress fiber assembly
G	0051668	biological_process	localization within membrane
G	0051894	biological_process	positive regulation of focal adhesion assembly
G	0055038	cellular_component	recycling endosome membrane
G	0060071	biological_process	Wnt signaling pathway, planar cell polarity pathway
G	0060091	cellular_component	kinocilium
G	0060263	biological_process	regulation of respiratory burst
G	0060326	biological_process	cell chemotaxis
G	0061344	biological_process	regulation of cell adhesion involved in heart morphogenesis
G	0070062	cellular_component	extracellular exosome
G	0071526	biological_process	semaphorin-plexin signaling pathway
G	0090023	biological_process	positive regulation of neutrophil chemotaxis
G	0097178	biological_process	ruffle assembly
G	0098794	cellular_component	postsynapse
G	0098978	cellular_component	glutamatergic synapse
G	0101003	cellular_component	ficolin-1-rich granule membrane
G	0150052	biological_process	regulation of postsynapse assembly
G	1900026	biological_process	positive regulation of substrate adhesion-dependent cell spreading
G	1900029	biological_process	positive regulation of ruffle assembly
G	1900242	biological_process	regulation of synaptic vesicle endocytosis
G	1902622	biological_process	regulation of neutrophil migration
G	1903348	biological_process	positive regulation of bicellular tight junction assembly
G	1904948	biological_process	midbrain dopaminergic neuron differentiation
G	2000386	biological_process	positive regulation of ovarian follicle development

Functional Information from SwissProt/UniProt

site_id	SWS_FT_FI1
Number of Residues	1
Details	Modified residue: {"description":"Phosphoserine","evidences":[{"source":"PubMed","id":"23186163","evidenceCode":"ECO:0007744"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI2
Number of Residues	20
Details	Transmembrane: {"description":"Helical","evidences":[{"evidenceCode":"ECO:0000255"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI3
Number of Residues	62
Details	Domain: {"description":"t-SNARE coiled-coil homology","evidences":[{"source":"PROSITE-ProRule","id":"PRU00202","evidenceCode":"ECO:0000255"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI4
Number of Residues	1
Details	Modified residue: {"description":"Phosphoserine","evidences":[{"source":"UniProtKB","id":"P62484","evidenceCode":"ECO:0000250"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI5
Number of Residues	16
Details	Motif: {"description":"Effector region","evidences":[{"evidenceCode":"ECO:0000255"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI6
Number of Residues	6
Details	Binding site: {"evidences":[{"source":"PubMed","id":"38332367","evidenceCode":"ECO:0000269"},{"source":"PDB","id":"8Q0N","evidenceCode":"ECO:0007744"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI7
Number of Residues	14
Details	Binding site: {"evidences":[{"source":"PubMed","id":"11090627","evidenceCode":"ECO:0000269"},{"source":"PubMed","id":"38332367","evidenceCode":"ECO:0000269"},{"source":"PubMed","id":"38355798","evidenceCode":"ECO:0000269"},{"source":"PDB","id":"1E96","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"2WKP","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"2WKQ","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"2WKR","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"8Q0N","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"8WEJ","evidenceCode":"ECO:0007744"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI8
Number of Residues	14
Details	Binding site: {"evidences":[{"source":"PubMed","id":"11090627","evidenceCode":"ECO:0000269"},{"source":"PubMed","id":"38332367","evidenceCode":"ECO:0000269"},{"source":"PDB","id":"1E96","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"2WKP","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"2WKQ","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"2WKR","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"8Q0N","evidenceCode":"ECO:0007744"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI9
Number of Residues	2
Details	Binding site: {"evidences":[{"source":"PubMed","id":"11090627","evidenceCode":"ECO:0000269"},{"source":"PDB","id":"1E96","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"2WKP","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"2WKQ","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"2WKR","evidenceCode":"ECO:0007744"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI10
Number of Residues	2
Details	Binding site: {"evidences":[{"source":"PubMed","id":"38355798","evidenceCode":"ECO:0000269"},{"source":"PDB","id":"8WEJ","evidenceCode":"ECO:0007744"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI11
Number of Residues	2
Details	Modified residue: {"description":"(Microbial infection) O-AMP-tyrosine; by Haemophilus IbpA; alternate","evidences":[{"source":"PubMed","id":"19362538","evidenceCode":"ECO:0000269"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI12
Number of Residues	2
Details	Modified residue: {"description":"(Microbial infection) O-AMP-threonine; by Vibrio VopS","evidences":[{"source":"PubMed","id":"19039103","evidenceCode":"ECO:0000269"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI13
Number of Residues	2
Details	Modified residue: {"description":"Phosphoserine","evidences":[{"source":"PubMed","id":"18669648","evidenceCode":"ECO:0007744"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI14
Number of Residues	2
Details	Glycosylation: {"description":"(Microbial infection) O-linked (GlcNAc) tyrosine; by Photorhabdus PAU_02230; alternate","evidences":[{"source":"PubMed","id":"24141704","evidenceCode":"ECO:0000269"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI15
Number of Residues	2
Details	Glycosylation: {"description":"(Microbial infection) O-linked (Glc) threonine; by C.difficile toxins TcdA and TcdB, and by P.sordellii toxin TcsL; alternate","evidences":[{"source":"PubMed","id":"19744486","evidenceCode":"ECO:0000269"},{"source":"PubMed","id":"24905543","evidenceCode":"ECO:0000269"},{"source":"PubMed","id":"7775453","evidenceCode":"ECO:0000269"},{"source":"PubMed","id":"7777059","evidenceCode":"ECO:0000269"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI16
Number of Residues	2
Details	Cross-link: {"description":"Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin)","evidences":[{"source":"PubMed","id":"18093184","evidenceCode":"ECO:0000269"},{"source":"PubMed","id":"38332367","evidenceCode":"ECO:0000269"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI17
Number of Residues	4
Details	Cross-link: {"description":"Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin)","evidences":[{"source":"PubMed","id":"23512198","evidenceCode":"ECO:0000269"}]}

Chain

Residue

Details

---