7USE
Cryo-EM structure of WAVE regulatory complex with Rac1 bound on both A and D site
EM specimen
| Vitrification | Yes |
| Instrument | HOMEMADE PLUNGER |
EM Data Collection
| Microscope | FEI TALOS ARCTICA |
| Electron Gun | FIELD EMISSION GUN |
| Accelerating Voltage (kV) | 200 |
| Cs (mm) | 2.7 |
| Detector | FEI FALCON III (4k x 4k) |
3D Reconstruction
| Method | SINGLE PARTICLE |
| Resolution (Å) | 3 |
| Number of Particle | 666417 |
| Software | PHENIX,cryoSPARC,EPU,NAMD,UCSF Chimera |
| Symmetry | C1 |
| CTF correction | PHASE FLIPPING AND AMPLITUDE CORRECTION |
