6E3L

Interferon gamma signalling complex with IFNGR1 and IFNGR2

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B (A, B)	Interferon gamma	polymer	148	17216.5	2	UniProt (P01579) Pfam (PF00714)	Homo sapiens (Human)	IFN-gamma,Immune interferon
2	C, D (C, D)	Interferon gamma receptor 1	polymer	242	27363.7	2	UniProt (P15260) Pfam (PF01108) Pfam (PF07140)	Homo sapiens (Human)	IFN-gamma-R1,CDw119,Interferon gamma receptor alpha-chain,IFN-gamma-R-alpha
3	E, F (E, I)	Interferon gamma receptor 2	polymer	233	26310.6	2	UniProt (P38484) Pfam (PF01108) Pfam (PF09294)	Homo sapiens (Human)	IFN-gamma-R2,Interferon gamma receptor accessory factor 1,AF-1,Interferon gamma receptor beta-chain,IFN-gamma-R-beta,Interferon gamma transducer 1
4	G, J, M (F, J, M)	2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose	branched		424.4	3	In PDB GlyTouCan (G42666HT)
5	H, I, K (G, H, K)	beta-D-mannopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose	branched		586.5	3	In PDB GlyTouCan (G15407YE)
6	L (L)	alpha-D-mannopyranose-(1-6)-alpha-D-mannopyranose-(1-6)-[alpha-D-mannopyranose-(1-3)]beta-D-mannopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose	branched		1073.0	1	In PDB GlyTouCan (G34442SS)
7	N, O, P, Q, R (A, B, D, E)	2-acetamido-2-deoxy-beta-D-glucopyranose	non-polymer		221.2	5	Chemie (NAG)
8	S, T (E, I)	CYSTEINE	non-polymer		121.2	2	Chemie (CYS)

Sequence modifications

A, B: 1 - 133 (UniProt: P01579)

PDB	External Database	Details
Gly -3	-	expression tag
Pro -2	-	expression tag
Gly -1	-	expression tag
Ser 0	-	expression tag
Ala 134	-	expression tag
Ala 135	-	expression tag
Ala 136	-	expression tag
His 137	-	expression tag
His 138	-	expression tag
His 139	-	expression tag
His 140	-	expression tag
His 141	-	expression tag
His 142	-	expression tag
His 143	-	expression tag
His 144	-	expression tag

C, D: 1 - 229 (UniProt: P15260)

PDB	External Database	Details
Gly -1	-	expression tag
Ser 0	-	expression tag
Ile 149	Thr 166	engineered mutation
Lys 161	Met 178	engineered mutation
Lys 167	Gln 184	engineered mutation
Asn 174	Lys 191	engineered mutation
Arg 182	Gln 199	engineered mutation
Asn 205	His 222	engineered mutation
Ala 230	-	expression tag
Ala 231	-	expression tag
Ala 232	-	expression tag
His 233	-	expression tag
His 234	-	expression tag
His 235	-	expression tag
His 236	-	expression tag
His 237	-	expression tag
His 238	-	expression tag
His 239	-	expression tag
His 240	-	expression tag

E, I: 28 - 247 (UniProt: P38484)

PDB	External Database	Details
Gly 26	-	expression tag
Ser 27	-	expression tag
Ala 248	-	expression tag
Ala 249	-	expression tag
Ala 250	-	expression tag
His 251	-	expression tag
His 252	-	expression tag
His 253	-	expression tag
His 254	-	expression tag
His 255	-	expression tag
His 256	-	expression tag
His 257	-	expression tag
His 258	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	6
	Total formula weight	141781.5
Branched	Number of molecules	7
	Total formula weight	4105.8
Non-Polymers*	Number of molecules	7
	Total formula weight	1348.4
All*	Total formula weight	147235.7

*Water molecules are not included.