6E3L

Interferon gamma signalling complex with IFNGR1 and IFNGR2

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 8.2.2
Synchrotron site	ALS
Beamline	8.2.2
Temperature [K]	100
Detector technology	CCD
Collection date	2017-09-26
Detector	ADSC QUANTUM 315
Wavelength(s)	0.999989
Spacegroup name	P 21 21 21
Unit cell lengths	78.694, 150.212, 212.668
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	48.738 - 3.800
R-factor	0.2493
Rwork	0.248
R-free	0.27240
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6e3k
RMSD bond length	0.004
RMSD bond angle	0.801
Data reduction software	XDS (Jan 26, 2018)
Data scaling software	Aimless (0.7.1)
Phasing software	PHASER (2.8.1)
Refinement software	PHENIX (1.14_3211)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	48.740	3.900
High resolution limit [Å]	3.800	3.800
Rmerge	0.271	3.524
Rmeas	0.281	3.649
Rpim	0.073	0.942
Number of reflections	25652	1875
<I/σ(I)>	10	0.9
Completeness [%]	99.9	100
Redundancy	14.6	14.9
CC(1/2)	0.999	0.429

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7	293	1.1 M Ammonium Tartrate dibasic pH 7.0