6D8C

Cryo-EM structure of FLNaABD E254K bound to phalloidin-stabilized F-actin

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, C, E, G, I (D, A, B, C, E)	Filamin-A	polymer	306	34476.3	5	UniProt (P21333) Pfam (PF00307)	Homo sapiens (Human)	FLN-A, Actin-binding protein 280, ABP-280, Alpha-filamin, Endothelial actin-binding protein, Filamin-1, Non-muscle filamin
2	B, D, F, H, J (H, J, K, L, M)	Actin, alpha skeletal muscle	polymer	375	41862.6	5	UniProt (P68139) Pfam (PF00022)	Gallus gallus (Chicken)	Alpha-actin-1
3	K, L, M (N, O, P)	Phalloidin	polymer	7	808.9	3	UniProt (by SIFTS) (P0CU65) BIRD (PRD_002307)	Amanita phalloides (Death cap)
4	N, P, R, T, V (H, J, K, L, M)	ADENOSINE-5'-DIPHOSPHATE	non-polymer		427.2	5	Chemie (ADP)
5	O, Q, S, U, W (H, J, K, L, M)	MAGNESIUM ION	non-polymer		24.3	5	Chemie (MG)

Sequence modifications

D, A, B, C, E: 1 - 278 (UniProt: P21333)

PDB	External Database	Details
Met -27	-	expression tag
His -26	-	expression tag
His -25	-	expression tag
His -24	-	expression tag
His -23	-	expression tag
His -22	-	expression tag
His -21	-	expression tag
Gly -20	-	expression tag
Ser -19	-	expression tag
Leu -18	-	expression tag
Val -17	-	expression tag
Pro -16	-	expression tag
Arg -15	-	expression tag
Ser -14	-	expression tag
Glu -13	-	expression tag
Asn -12	-	expression tag
Leu -11	-	expression tag
Tyr -10	-	expression tag
Phe -9	-	expression tag
Gln -8	-	expression tag
Gly -7	-	expression tag
Ser -6	-	expression tag
Asp -5	-	expression tag
Ile -4	-	expression tag
Leu -3	-	expression tag
Gln -2	-	expression tag
Gly -1	-	expression tag
Thr 0	-	expression tag
Lys 254	Glu 254	engineered mutation

Contents of the asymmetric unit

Polymers	Number of chains	13
	Total formula weight	384121.0
Non-Polymers*	Number of molecules	10
	Total formula weight	2257.5
All*	Total formula weight	386378.5

*Water molecules are not included.