5TSU

Active conformation for Engineered human cystathionine gamma lyase (E59N, R119L, E339V) to depleting methionine

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B, D, F, G... (A, B, D, F, G...)	Cystathionine gamma-lyase	polymer	422	46584.0	6	UniProt (P32929) Pfam (PF01053)	Homo sapiens (Human)	Cysteine-protein sulfhydrase,Gamma-cystathionase
2	C, E (C, E)	Cystathionine gamma-lyase	polymer	422	46355.9	2	UniProt (P32929) Pfam (PF01053)	Homo sapiens (Human)	Cysteine-protein sulfhydrase,Gamma-cystathionase
3	I (A)	METHIONINE	non-polymer		149.2	1	Chemie (MET)
4	J, K, M (A, B, D)	SULFATE ION	non-polymer		96.1	3	Chemie (SO4)
5	L, N (C, E)	N-({3-hydroxy-2-methyl-5-[(phosphonooxy)methyl]pyridin-4-yl}methyl)-L-methionine	non-polymer		380.4	2	Chemie (LPI)
6	O, P, Q (F, G, H)	CYSTEINE	non-polymer		121.2	3	Chemie (CYS)
7	R, S, T, U, V... (A, B, C, D, E...)	water	water		18.0	1012	Chemie (HOH)

Sequence modifications

A, B, D, F, G, H: 2 - 405 (UniProt: P32929)

PDB	External Database	Details
Met -16	-	expression tag
Gly -15	-	expression tag
Gly -14	-	expression tag
His -13	-	expression tag
His -12	-	expression tag
His -11	-	expression tag
His -10	-	expression tag
His -9	-	expression tag
His -8	-	expression tag
Gly -7	-	expression tag
Leu -6	-	expression tag
Glu -5	-	expression tag
Val -4	-	expression tag
Leu -3	-	expression tag
Phe -2	-	expression tag
Gln -1	-	expression tag
Gly 0	-	expression tag
Pro 1	-	expression tag
Asn 59	Glu 59	engineered mutation
Leu 119	Arg 119	engineered mutation
Val 339	Glu 339	engineered mutation

C, E: 2 - 405 (UniProt: P32929)

PDB	External Database	Details
Met -16	-	initiating methionine
Gly -15	-	expression tag
Gly -14	-	expression tag
His -13	-	expression tag
His -12	-	expression tag
His -11	-	expression tag
His -10	-	expression tag
His -9	-	expression tag
His -8	-	expression tag
Gly -7	-	expression tag
Leu -6	-	expression tag
Glu -5	-	expression tag
Val -4	-	expression tag
Leu -3	-	expression tag
Phe -2	-	expression tag
Gln -1	-	expression tag
Gly 0	-	expression tag
Pro 1	-	expression tag
Asn 59	Glu 59	engineered mutation
Leu 119	Arg 119	engineered mutation
Val 339	Glu 339	engineered mutation

Contents of the asymmetric unit

Polymers	Number of chains	8
	Total formula weight	372216.0
Non-Polymers*	Number of molecules	9
	Total formula weight	1561.6
All*	Total formula weight	373777.6

*Water molecules are not included.