5TSU

Active conformation for Engineered human cystathionine gamma lyase (E59N, R119L, E339V) to depleting methionine

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 5.0.3
Synchrotron site	ALS
Beamline	5.0.3
Temperature [K]	100
Detector technology	CCD
Collection date	2015-10-09
Detector	ADSC QUANTUM 315r
Wavelength(s)	0.97741
Spacegroup name	P 2 21 21
Unit cell lengths	111.889, 163.683, 181.099
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	49.041 - 2.200
R-factor	0.1725
Rwork	0.171
R-free	0.20170
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2nmp
RMSD bond length	0.004
RMSD bond angle	0.703
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX ((1.10.1_2155: ???))

Data quality characteristics

	Overall
Low resolution limit [Å]	50.000
High resolution limit [Å]	2.200
Rmerge	0.097
Number of reflections	161429
<I/σ(I)>	18.784
Completeness [%]	95.9
Redundancy	5.1

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	6.5	277	100mM Sodium Cocadylate PH6.5 150mM Sodium Cirtate 305 Isopropanol