Summary Structural details Experimental details Functional details Sequence Neighbor History Downloads

5TSU

Active conformation for Engineered human cystathionine gamma lyase (E59N, R119L, E339V) to depleting methionine

Functional Information from GO Data

Chain	GOid	namespace	contents
A	0004123	molecular_function	cystathionine gamma-lyase activity
A	0005515	molecular_function	protein binding
A	0005516	molecular_function	calmodulin binding
A	0005737	cellular_component	cytoplasm
A	0005829	cellular_component	cytosol
A	0006534	biological_process	cysteine metabolic process
A	0006629	biological_process	lipid metabolic process
A	0016829	molecular_function	lyase activity
A	0018272	biological_process	protein-pyridoxal-5-phosphate linkage via peptidyl-N6-pyridoxal phosphate-L-lysine
A	0019343	biological_process	cysteine biosynthetic process via cystathionine
A	0019344	biological_process	cysteine biosynthetic process
A	0019346	biological_process	transsulfuration
A	0030170	molecular_function	pyridoxal phosphate binding
A	0030968	biological_process	endoplasmic reticulum unfolded protein response
A	0042802	molecular_function	identical protein binding
A	0043066	biological_process	negative regulation of apoptotic process
A	0043123	biological_process	positive regulation of canonical NF-kappaB signal transduction
A	0044524	biological_process	protein sulfhydration
A	0044540	molecular_function	L-cystine L-cysteine-lyase (deaminating)
A	0047982	molecular_function	homocysteine desulfhydrase activity
A	0051092	biological_process	positive regulation of NF-kappaB transcription factor activity
A	0051289	biological_process	protein homotetramerization
A	0070062	cellular_component	extracellular exosome
A	0070814	biological_process	hydrogen sulfide biosynthetic process
A	0080146	molecular_function	L-cysteine desulfhydrase activity
A	0098606	molecular_function	selenocystathionine gamma-lyase activity
A	1904831	biological_process	positive regulation of aortic smooth muscle cell differentiation
A	1990830	biological_process	cellular response to leukemia inhibitory factor
A	2001234	biological_process	negative regulation of apoptotic signaling pathway
B	0004123	molecular_function	cystathionine gamma-lyase activity
B	0005515	molecular_function	protein binding
B	0005516	molecular_function	calmodulin binding
B	0005737	cellular_component	cytoplasm
B	0005829	cellular_component	cytosol
B	0006534	biological_process	cysteine metabolic process
B	0006629	biological_process	lipid metabolic process
B	0016829	molecular_function	lyase activity
B	0018272	biological_process	protein-pyridoxal-5-phosphate linkage via peptidyl-N6-pyridoxal phosphate-L-lysine
B	0019343	biological_process	cysteine biosynthetic process via cystathionine
B	0019344	biological_process	cysteine biosynthetic process
B	0019346	biological_process	transsulfuration
B	0030170	molecular_function	pyridoxal phosphate binding
B	0030968	biological_process	endoplasmic reticulum unfolded protein response
B	0042802	molecular_function	identical protein binding
B	0043066	biological_process	negative regulation of apoptotic process
B	0043123	biological_process	positive regulation of canonical NF-kappaB signal transduction
B	0044524	biological_process	protein sulfhydration
B	0044540	molecular_function	L-cystine L-cysteine-lyase (deaminating)
B	0047982	molecular_function	homocysteine desulfhydrase activity
B	0051092	biological_process	positive regulation of NF-kappaB transcription factor activity
B	0051289	biological_process	protein homotetramerization
B	0070062	cellular_component	extracellular exosome
B	0070814	biological_process	hydrogen sulfide biosynthetic process
B	0080146	molecular_function	L-cysteine desulfhydrase activity
B	0098606	molecular_function	selenocystathionine gamma-lyase activity
B	1904831	biological_process	positive regulation of aortic smooth muscle cell differentiation
B	1990830	biological_process	cellular response to leukemia inhibitory factor
B	2001234	biological_process	negative regulation of apoptotic signaling pathway
C	0004123	molecular_function	cystathionine gamma-lyase activity
C	0005515	molecular_function	protein binding
C	0005516	molecular_function	calmodulin binding
C	0005737	cellular_component	cytoplasm
C	0005829	cellular_component	cytosol
C	0006534	biological_process	cysteine metabolic process
C	0006629	biological_process	lipid metabolic process
C	0016829	molecular_function	lyase activity
C	0018272	biological_process	protein-pyridoxal-5-phosphate linkage via peptidyl-N6-pyridoxal phosphate-L-lysine
C	0019343	biological_process	cysteine biosynthetic process via cystathionine
C	0019344	biological_process	cysteine biosynthetic process
C	0019346	biological_process	transsulfuration
C	0030170	molecular_function	pyridoxal phosphate binding
C	0030968	biological_process	endoplasmic reticulum unfolded protein response
C	0042802	molecular_function	identical protein binding
C	0043066	biological_process	negative regulation of apoptotic process
C	0043123	biological_process	positive regulation of canonical NF-kappaB signal transduction
C	0044524	biological_process	protein sulfhydration
C	0044540	molecular_function	L-cystine L-cysteine-lyase (deaminating)
C	0047982	molecular_function	homocysteine desulfhydrase activity
C	0051092	biological_process	positive regulation of NF-kappaB transcription factor activity
C	0051289	biological_process	protein homotetramerization
C	0070062	cellular_component	extracellular exosome
C	0070814	biological_process	hydrogen sulfide biosynthetic process
C	0080146	molecular_function	L-cysteine desulfhydrase activity
C	0098606	molecular_function	selenocystathionine gamma-lyase activity
C	1904831	biological_process	positive regulation of aortic smooth muscle cell differentiation
C	1990830	biological_process	cellular response to leukemia inhibitory factor
C	2001234	biological_process	negative regulation of apoptotic signaling pathway
D	0004123	molecular_function	cystathionine gamma-lyase activity
D	0005515	molecular_function	protein binding
D	0005516	molecular_function	calmodulin binding
D	0005737	cellular_component	cytoplasm
D	0005829	cellular_component	cytosol
D	0006534	biological_process	cysteine metabolic process
D	0006629	biological_process	lipid metabolic process
D	0016829	molecular_function	lyase activity
D	0018272	biological_process	protein-pyridoxal-5-phosphate linkage via peptidyl-N6-pyridoxal phosphate-L-lysine
D	0019343	biological_process	cysteine biosynthetic process via cystathionine
D	0019344	biological_process	cysteine biosynthetic process
D	0019346	biological_process	transsulfuration
D	0030170	molecular_function	pyridoxal phosphate binding
D	0030968	biological_process	endoplasmic reticulum unfolded protein response
D	0042802	molecular_function	identical protein binding
D	0043066	biological_process	negative regulation of apoptotic process
D	0043123	biological_process	positive regulation of canonical NF-kappaB signal transduction
D	0044524	biological_process	protein sulfhydration
D	0044540	molecular_function	L-cystine L-cysteine-lyase (deaminating)
D	0047982	molecular_function	homocysteine desulfhydrase activity
D	0051092	biological_process	positive regulation of NF-kappaB transcription factor activity
D	0051289	biological_process	protein homotetramerization
D	0070062	cellular_component	extracellular exosome
D	0070814	biological_process	hydrogen sulfide biosynthetic process
D	0080146	molecular_function	L-cysteine desulfhydrase activity
D	0098606	molecular_function	selenocystathionine gamma-lyase activity
D	1904831	biological_process	positive regulation of aortic smooth muscle cell differentiation
D	1990830	biological_process	cellular response to leukemia inhibitory factor
D	2001234	biological_process	negative regulation of apoptotic signaling pathway
E	0004123	molecular_function	cystathionine gamma-lyase activity
E	0005515	molecular_function	protein binding
E	0005516	molecular_function	calmodulin binding
E	0005737	cellular_component	cytoplasm
E	0005829	cellular_component	cytosol
E	0006534	biological_process	cysteine metabolic process
E	0006629	biological_process	lipid metabolic process
E	0016829	molecular_function	lyase activity
E	0018272	biological_process	protein-pyridoxal-5-phosphate linkage via peptidyl-N6-pyridoxal phosphate-L-lysine
E	0019343	biological_process	cysteine biosynthetic process via cystathionine
E	0019344	biological_process	cysteine biosynthetic process
E	0019346	biological_process	transsulfuration
E	0030170	molecular_function	pyridoxal phosphate binding
E	0030968	biological_process	endoplasmic reticulum unfolded protein response
E	0042802	molecular_function	identical protein binding
E	0043066	biological_process	negative regulation of apoptotic process
E	0043123	biological_process	positive regulation of canonical NF-kappaB signal transduction
E	0044524	biological_process	protein sulfhydration
E	0044540	molecular_function	L-cystine L-cysteine-lyase (deaminating)
E	0047982	molecular_function	homocysteine desulfhydrase activity
E	0051092	biological_process	positive regulation of NF-kappaB transcription factor activity
E	0051289	biological_process	protein homotetramerization
E	0070062	cellular_component	extracellular exosome
E	0070814	biological_process	hydrogen sulfide biosynthetic process
E	0080146	molecular_function	L-cysteine desulfhydrase activity
E	0098606	molecular_function	selenocystathionine gamma-lyase activity
E	1904831	biological_process	positive regulation of aortic smooth muscle cell differentiation
E	1990830	biological_process	cellular response to leukemia inhibitory factor
E	2001234	biological_process	negative regulation of apoptotic signaling pathway
F	0004123	molecular_function	cystathionine gamma-lyase activity
F	0005515	molecular_function	protein binding
F	0005516	molecular_function	calmodulin binding
F	0005737	cellular_component	cytoplasm
F	0005829	cellular_component	cytosol
F	0006534	biological_process	cysteine metabolic process
F	0006629	biological_process	lipid metabolic process
F	0016829	molecular_function	lyase activity
F	0018272	biological_process	protein-pyridoxal-5-phosphate linkage via peptidyl-N6-pyridoxal phosphate-L-lysine
F	0019343	biological_process	cysteine biosynthetic process via cystathionine
F	0019344	biological_process	cysteine biosynthetic process
F	0019346	biological_process	transsulfuration
F	0030170	molecular_function	pyridoxal phosphate binding
F	0030968	biological_process	endoplasmic reticulum unfolded protein response
F	0042802	molecular_function	identical protein binding
F	0043066	biological_process	negative regulation of apoptotic process
F	0043123	biological_process	positive regulation of canonical NF-kappaB signal transduction
F	0044524	biological_process	protein sulfhydration
F	0044540	molecular_function	L-cystine L-cysteine-lyase (deaminating)
F	0047982	molecular_function	homocysteine desulfhydrase activity
F	0051092	biological_process	positive regulation of NF-kappaB transcription factor activity
F	0051289	biological_process	protein homotetramerization
F	0070062	cellular_component	extracellular exosome
F	0070814	biological_process	hydrogen sulfide biosynthetic process
F	0080146	molecular_function	L-cysteine desulfhydrase activity
F	0098606	molecular_function	selenocystathionine gamma-lyase activity
F	1904831	biological_process	positive regulation of aortic smooth muscle cell differentiation
F	1990830	biological_process	cellular response to leukemia inhibitory factor
F	2001234	biological_process	negative regulation of apoptotic signaling pathway
G	0004123	molecular_function	cystathionine gamma-lyase activity
G	0005515	molecular_function	protein binding
G	0005516	molecular_function	calmodulin binding
G	0005737	cellular_component	cytoplasm
G	0005829	cellular_component	cytosol
G	0006534	biological_process	cysteine metabolic process
G	0006629	biological_process	lipid metabolic process
G	0016829	molecular_function	lyase activity
G	0018272	biological_process	protein-pyridoxal-5-phosphate linkage via peptidyl-N6-pyridoxal phosphate-L-lysine
G	0019343	biological_process	cysteine biosynthetic process via cystathionine
G	0019344	biological_process	cysteine biosynthetic process
G	0019346	biological_process	transsulfuration
G	0030170	molecular_function	pyridoxal phosphate binding
G	0030968	biological_process	endoplasmic reticulum unfolded protein response
G	0042802	molecular_function	identical protein binding
G	0043066	biological_process	negative regulation of apoptotic process
G	0043123	biological_process	positive regulation of canonical NF-kappaB signal transduction
G	0044524	biological_process	protein sulfhydration
G	0044540	molecular_function	L-cystine L-cysteine-lyase (deaminating)
G	0047982	molecular_function	homocysteine desulfhydrase activity
G	0051092	biological_process	positive regulation of NF-kappaB transcription factor activity
G	0051289	biological_process	protein homotetramerization
G	0070062	cellular_component	extracellular exosome
G	0070814	biological_process	hydrogen sulfide biosynthetic process
G	0080146	molecular_function	L-cysteine desulfhydrase activity
G	0098606	molecular_function	selenocystathionine gamma-lyase activity
G	1904831	biological_process	positive regulation of aortic smooth muscle cell differentiation
G	1990830	biological_process	cellular response to leukemia inhibitory factor
G	2001234	biological_process	negative regulation of apoptotic signaling pathway
H	0004123	molecular_function	cystathionine gamma-lyase activity
H	0005515	molecular_function	protein binding
H	0005516	molecular_function	calmodulin binding
H	0005737	cellular_component	cytoplasm
H	0005829	cellular_component	cytosol
H	0006534	biological_process	cysteine metabolic process
H	0006629	biological_process	lipid metabolic process
H	0016829	molecular_function	lyase activity
H	0018272	biological_process	protein-pyridoxal-5-phosphate linkage via peptidyl-N6-pyridoxal phosphate-L-lysine
H	0019343	biological_process	cysteine biosynthetic process via cystathionine
H	0019344	biological_process	cysteine biosynthetic process
H	0019346	biological_process	transsulfuration
H	0030170	molecular_function	pyridoxal phosphate binding
H	0030968	biological_process	endoplasmic reticulum unfolded protein response
H	0042802	molecular_function	identical protein binding
H	0043066	biological_process	negative regulation of apoptotic process
H	0043123	biological_process	positive regulation of canonical NF-kappaB signal transduction
H	0044524	biological_process	protein sulfhydration
H	0044540	molecular_function	L-cystine L-cysteine-lyase (deaminating)
H	0047982	molecular_function	homocysteine desulfhydrase activity
H	0051092	biological_process	positive regulation of NF-kappaB transcription factor activity
H	0051289	biological_process	protein homotetramerization
H	0070062	cellular_component	extracellular exosome
H	0070814	biological_process	hydrogen sulfide biosynthetic process
H	0080146	molecular_function	L-cysteine desulfhydrase activity
H	0098606	molecular_function	selenocystathionine gamma-lyase activity
H	1904831	biological_process	positive regulation of aortic smooth muscle cell differentiation
H	1990830	biological_process	cellular response to leukemia inhibitory factor
H	2001234	biological_process	negative regulation of apoptotic signaling pathway

Functional Information from PDB Data

site_id	AC1
Number of Residues	7
Details	binding site for residue MET A 501

Chain	Residue
A	TYR114
A	ASN161
A	LLP212
A	SER340
A	ARG375
B	ASN59
B	TYR60

site_id	AC2
Number of Residues	4
Details	binding site for residue SO4 A 502

Chain	Residue
A	HOH631
C	LYS139
A	LYS165
A	GLN299

site_id	AC3
Number of Residues	7
Details	binding site for residue SO4 B 501

Chain	Residue
B	TYR114
B	ASN161
B	LLP212
B	SER340
B	LEU341
B	THR355
B	ARG375

site_id	AC4
Number of Residues	6
Details	binding site for residue SO4 D 501

Chain	Residue
D	ASN161
D	LLP212
D	SER340
D	GLU349
D	THR355
D	ARG375

site_id	AC5
Number of Residues	17
Details	binding site for residue LPI E 501

Chain	Residue
E	SER89
E	GLY90
E	LEU91
E	TYR114
E	GLU157
E	ASN161
E	ASP187
E	THR189
E	SER209
E	THR211
E	LYS212
E	VAL339
E	SER340
E	THR355
E	ARG375
F	TYR60
F	ARG62

site_id	AC6
Number of Residues	8
Details	binding site for residue CYS F 501

Chain	Residue
F	TYR114
F	ASN161
F	LLP212
F	SER340
F	LEU341
F	GLU349
F	THR355
F	ARG375

site_id	AC7
Number of Residues	7
Details	binding site for residue CYS G 501

Chain	Residue
G	TYR114
G	ASN161
G	LLP212
G	SER340
G	THR355
G	ARG375
H	TYR60

site_id	AC8
Number of Residues	8
Details	binding site for residue CYS H 501

Chain	Residue
G	TYR60
H	TYR114
H	ASN161
H	LLP212
H	VAL339
H	SER340
H	THR355
H	ARG375

site_id	AC9
Number of Residues	22
Details	binding site for Di-peptide LPI C 501 and LYS C 212

Chain	Residue
C	SER89
C	GLY90
C	LEU91
C	TYR114
C	GLU157
C	ASN161
C	ASP187
C	THR189
C	SER209
C	ALA210
C	THR211
C	TYR213
C	MET214
C	GLY216
C	SER340
C	LEU341
C	GLY342
C	THR355
C	ARG375
C	HOH697
D	TYR60
D	ARG62

Functional Information from PROSITE/UniProt

site_id	PS00868
Number of Residues	15
Details	CYS_MET_METAB_PP Cys/Met metabolism enzymes pyridoxal-phosphate attachment site. DIsmySATKYMnGHS

Chain	Residue	Details
A	ASP204-SER218
C	ASP204-SER218

Functional Information from SwissProt/UniProt

site_id	SWS_FT_FI1
Number of Residues	8
Details	BINDING:

Chain	Residue	Details
C	ARG62
D	TYR114
D	LEU119
D	VAL339
F	ARG62
F	TYR114
F	LEU119
F	VAL339
G	ARG62
G	TYR114
G	LEU119
C	TYR114
G	VAL339
H	ARG62
H	TYR114
H	LEU119
H	VAL339
C	LEU119
C	VAL339
E	ARG62
E	TYR114
E	LEU119
E	VAL339
D	ARG62

site_id	SWS_FT_FI2
Number of Residues	2
Details	MOD_RES: N6-(pyridoxal phosphate)lysine => ECO:0000269\|PubMed:19019829

Chain	Residue	Details
C	LYS212
E	LYS212
D	LLP212
F	LLP212
G	LLP212
H	LLP212

		Sequence (fasta)
		PDBx/mmCIF
		PDBML format (no-atom)
		PDB format (full)
		Validation report (PDF)
		EDMap 2fo-fc (MTZ)