5OFO

Cryo EM structure of the E. coli disaggregase ClpB (BAP form, DWB mutant), in the ATPgammaS state, bound to the model substrate casein

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	C, F, E, D, B...	Chaperone protein ClpB,ATP-dependent Clp protease ATP-binding subunit ClpA,Chaperone protein ClpB	polymer	871	97288.9	6	UniProt (P63284) UniProt (P0ABH9) Pfam (PF02861) Pfam (PF00004) Pfam (PF17871) Pfam (PF07724) Pfam (PF10431) In PDB	Escherichia coli (strain K12)	Heat shock protein F84.1,Heat shock protein F84.1
2	C, F, E, D, B...	PHOSPHOTHIOPHOSPHORIC ACID-ADENYLATE ESTER	non-polymer		523.2	11	Chemie (AGS)

Sequence modifications

C, F, E, D, B, A: 1 - 721 (UniProt: P63284)

PDB	External Database	Details
Met -3	-	initiating methionine
Arg -2	-	expression tag
Gly -1	-	expression tag
Ser 0	-	expression tag
Ala 279	Glu 279	engineered mutation
Ala 678	Glu 678	engineered mutation

C, F, E, D, B, A: 722 - 748 (UniProt: P0ABH9)

PDB	External Database	Details
Ile 745	Ile 632	linker
Lys 746	Lys 633	linker
Lys 747	Lys 634	linker
Ile 748	Ile 635	linker

C, F, E, D, B, A: 749 - 857 (UniProt: P63284)

PDB	External Database	Details
Gly 858	-	expression tag
Ser 859	-	expression tag
Arg 860	-	expression tag
Ser 861	-	expression tag
His 862	-	expression tag
His 863	-	expression tag
His 864	-	expression tag
His 865	-	expression tag
His 866	-	expression tag
His 867	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	6
	Total formula weight	583733.5
Non-Polymers*	Number of molecules	11
	Total formula weight	5755.7
All*	Total formula weight	589489.2

*Water molecules are not included.