5OFO
Cryo EM structure of the E. coli disaggregase ClpB (BAP form, DWB mutant), in the ATPgammaS state, bound to the model substrate casein
EM specimen
| Vitrification | Yes |
| Instrument | FEI VITROBOT MARK III |
EM Data Collection
| Microscope | FEI TITAN KRIOS |
| Electron Gun | FIELD EMISSION GUN |
| Accelerating Voltage (kV) | 300 |
| Detector | GATAN K2 SUMMIT (4k x 4k) |
3D Reconstruction
| Method | SINGLE PARTICLE |
| Resolution (Å) | 4.6 |
| Number of Particle | 500000 |
| Software | Gautomatch,RELION,PHENIX,EPU,CTFFIND,iMODFIT,Flex-EM |
| Symmetry | C1 |
| CTF correction | PHASE FLIPPING AND AMPLITUDE CORRECTION |
